Mitsubishi Petrochemical Company Limited

Seiichi Kawarabuki

Naoto Miwa

Toshiyuki Sai

Yoshiro Masuda

Masanobu Inagawa

Takao Saito

A method and composition for use therein for measuring the activity of creatine kinase (hereinafter referred to as 'CK') having good storage stability is disclosed. More particularly, a composition for measurement of CK activity which comprises as main components phosphoglycerate kinase (hereinafter referred to a 'PGK') which catalyzes the following reaction 2 and glyceraldehyde-3-phosphate dehydrogenase (hereinafter referred to as 'GAPDH') which catalyzes the following reaction 3. Reaction 1 is catalyzed by the creatine kinase.

Method of measuring creatine kinase activity

This invention relates to a process for producing collagenase, which comprises aerobically cultivating a collagenase-producing bacterium belonging to the genus Bacillus, and recovering collagenase from the culture broth. This invention also provides a thermophilic bacterium of the genus Bacillus which has the ability to produce collagenase and which grows at a temperature of 42.degree. to 74.degree. C. and a pH of 5.0 to 9.0 and a novel thermophilic collagenase which does not substantially lose collagenase activity even when maintained for 1 hour at a temperature of 60.degree. C. in the presence of a calcium ion at a pH in the range of 6.5 to 8.5, and which has a molecular weight, determined by gel filtration, between those of cytochrome C and ovalbumin.

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Ibaraki, Japan

Seiichi Kawarabuki