Ajinomoto Co., Ltd.

Seiichi Hara

Kenzo Yokozeki

Isao Abe

Naoto Tonouchi

Yasuko Jojima

Ayako Ohno

Shunichi Suzuki

Yasuaki Takakura

Toshiki Taba

Kunihiko Watanabe

Masakazu Sugiyama

Sonoko Suzuki

DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genusand having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

Enzyme that catalyzes a peptide-forming reaction from a carboxy component and an amine component, microbe producing the same, and a method of producing a dipeptide using the enzyme or microbe

It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

Methods for making 3-indole-pyruvic acid from tryptophan using a tryptophan deaminase

Oxidase gene and method for producing 3-indole-pyruvic acid by utilizing the gene

A method of producing an &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b2;-ester by forming the &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b2;-ester from L-aspartic acid-&#x3b1;,&#x3b2;-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an &#x3b1;-ester site of the L-aspartic acid-&#x3b1;,&#x3b2;-diester through a peptide bond.

Method for producing &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b2;-ester and method for producing &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b1;-methyl ester

The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an amino acid ester and an amino acid, microbial cells separated from the culture, or treated microbial cell product.

Method for producing dipeptides

Method for producing a dipeptide

A method of producing an &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b2;-ester by forming the &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b2;-ester from L-aspartic acid-&#x3b1;&#x3b2;-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an &#x3b1;-ester site of the L-aspartic acid-&#x3b1;&#x3b2;-diester through a peptide bond.

A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genusor the genusis provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.

Method for producing tripeptides and/or peptides longer than tripeptides

A method for producing an &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b2;-ester (also named &#x3b1;-L-(&#x3b2;-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b1;-methyl ester (also named &#x3b1;-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b1;-methyl ester is provided. &#x3b1;-L-aspartyl-L-phenylalanine-&#x3b1;-methyl ester is produced from a L-aspartic acid-&#x3b1;,&#x3b2;-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a &#x3b2;-ester site of L-aspartic acid-&#x3b1;,&#x3b2;-diester but performs a nucleophilic attack on an &#x3b1;-ester site thereof.

Growing community of inventors

Kawasaki, Japan

Seiichi Hara