Osong Medical Innovation Foundation, Korea University Research and Business Foundation

Sehyun Shin

Chang-Soo Han

Won-Hwi Na

Dae Ho Jang

Hwang-Soo Kim

Wonhwi Na

Ye Eun Oh

Daeho Jang

Ho Yoon Lee

Jin-Hyun Kim

The present invention relates to a method of detecting a target nucleic acid on the basis of rolling circle amplification (RCA), and more specifically, to a method of detecting a target nucleic acid, the method in which a target nucleic acid (a nucleic acid having a target nucleic acid sequence), when present, forms a circular template with a template for performing an amplification reaction, wherein during the amplification reaction, a restriction enzyme is added to further induce a new RCA reaction, thus increasing the reaction rate and sensitivity, and to an RCA composition for implementing the method. The method of detecting a target nucleic acid according to the present invention, by detecting a barcode sequence predefined according to the type of the target nucleic acid, enables multiple detections of the presence of the target nucleic acid without sequencing, is inexpensive for not using costly enzymes, such as CRISPR, can detect barcode sequences, and can utilize various existing nucleic acid detection systems, and thus, can be useful in the detection of gene mutations.

Method of detecting target nucleic acid using rolling circle amplification and composition for detecting target nucleic acid

Disclosed are a gene detection device including gold nanoparticles and a gene detection method using gold nanoparticles. The use of the gene detection device and the gene detection method avoids the need for special equipment, such as a thermal cycler, which is essential for 3-stage heating in conventional PCR-based gene amplification techniques. In addition, the gene detection device and the gene detection method enable rapid and sensitive quantitative analysis and multiple detection.

Gene detection device including gold nanoparticles and gene detection method using gold nanoparticles

The present invention relates to an apparatus and method for detecting and enumerating a rare cell in blood. The apparatus comprises a sample collector configured to collect a blood sample and receive a complex in which a target antibody and a marker are coupled; a measuring kit connected to the sample collector in such a way that a mixture of the blood sample and the complex is injected into the measuring kit, thereby individually trapping a blood cell; and a detector configured to detect and enumerate the rare cell having an antigen-antibody reaction with the target antibody among the blood cell trapped in the measuring kit.

Apparatus and method for detecting and counting rare cells in blood

Growing community of inventors

Seoul, South Korea

Sehyun Shin