Roche Sequencing Solutions, Inc.

Ventana Medical Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Sedide Ozturk

Brian Christopher Godwin

Jan Berka

Seoyoung Kim

Joseph Platzer

Carolina Dallett

Florian Rubelt

Khai Luong

Richard Dannebaum

Martin Ranik

Jainee Lewis

Larry E Morrison

Jigar Patel

Nelson Alexander

Stacey Stanislaw

Aoune Barhoumi

Rui Chen

Toumy Guettouche

Lisa L Gallegos

Maeve E O'Huallachain

Dilduz Telman

Toummy Guettouche

Nelson R Alexander

Maeve E O'HUALLACHAIN

The invention is a method of single cell transcriptome analysis. The method comprises detecting multiple transcripts in each individual cell of the plurality of cells by barcoding the transcripts with a cell-specific compound barcode formed using a DNA polymerase and a terminal transferase, optionally in a single enzyme such as a reverse transcriptase.

Whole transcriptome analysis in single cells

The present invention is a method and compositions for primer extension target enrichment of nucleic acids and improvements thereto including simultaneously enriching for RNA and DNA and optionally sequencing the enriched products.

Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA

Methods and compositions are described herein for primer extension target enrichment of immune receptor (BCR or TCR) sequences.

Immune profiling by primer extension target enrichment

Immuno-PETE

The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

System for identification of antigens recognized by T cell receptors expressed on tumor infiltrating lymphocytes

The present invention is a method and compositions for primer extension target enrichment of nucleic acids and improvements thereto including simultaneously enriching for RNA and DNA and optionally sequencing the enriched products. An embodiment of the present invention includes a method comprising the steps of: hybridizing a target-specific primer to a target DNA or RNA, wherein the primer comprises a target-binding region and a region of complementarity to an adaptor; extending the primer with a DNA polymerase or reverse transcriptase to form a primer extension product; contacting the product with an adaptor comprising a longer strand with a 5'-overhang having complementarity to said primer and a shorter strand comprising a universal priming site; hybridizing the adaptor to the product; and ligating one strand of the adaptor to the product to form a ligation product.

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Pleasanton, CA, United States of America

Sedide Ozturk