Cytoo

Sebastien Degot

Yoran Margaron

Mathieu Fernandes

Joris Michaud

Alexandra Fuchs

Pauline Poydenot

Eve Duchemin-Pelletier

Michel Bornens

Maria Luisa Calvo Munoz

Amelie Argento-Pucciarelli

Aurelie Berthelot

Joanne Young

Delphine Morales

Jie Liu

The invention relates to a method for high throughput screening compounds by using a plurality of automated cell based assays assessing skeletal muscle cells contractility, morphology, and metabolism, in order to predict the efficacy of said compound on a panel of applications linked with muscle physiological and pathophysiological processes, comprising: (i) providing an in vitro culture of myotubes, wherein the in vitro myotubes culture is obtained by the following method: providing a cell culture device allowing the culture of myoblasts or myotubes, depositing said cells from a human donor or human group of donors, in good health or affected by a muscle related disorder, from primary cells, a cell line, an isogenic cell line or differentiated stem cells recapitulating a muscle disorder, on said culture device by using a method allowing the spatial control of cell culture, culturing said cells during a determined incubation time so as to promote a spatially controlled myotube culture; (ii) adding at least one compound to said culture; (iii) after a determined incubation time of the myotubes with said compound, carrying out structural and/or functional readouts of the myotubes to determine the effect of said compound on the myotubes; and (iv) based on said determined effect, predicting the ability of said compound to improve or alter healthy muscle features, or to treat, rescue, or cure muscle disorders, said features or said disorders being linked with muscle contraction, muscle morphology or muscle metabolism.

High throughput and functional screening method for muscle related disorders and biological processes

A method and device for standardizing myoblast differentiation into myotubes, including a substrate () and at least one cell-adhesive pattern () for culturing myoblasts on the substrate. The pattern () has an elongated surface. A central region (C) and two lateral regions (L) extend from the central region in both directions along a longitudinal axis of the pattern. The ratio between the maximum width (W) of the central region (C) and the maximum width (W) of the lateral regions (L) is greater than or equal to 2. The ratio between the length (L) and the maximum width (W) of the pattern () is less than or equal to 4. The method includes providing a device as described above, depositing myoblasts on at least one cell-adhesive pattern of the device, culturing the myoblasts in a differentiation medium to promote cell differentiation into myotubes and constrain elongation of the myotubes.

Device and method for standardizing myoblast differentiation into myotubes

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Grenoble, France

Sebastien Degot