Hoffmann-la Roche Inc.

Roche Palo Alto LLC

F. Hoffmann-la Roche Ag

Lsp - Hamburg Ug - Haftungsbeschränkt

Acmos Chemie Kg

Sebastian Neumann

Hubert Kettenberger

Stefan Klostermann

Ulrich Kohnert

Paul Parren

Stefan Seeber

Joerg Thomas Regula

Jan Van De Winkel

Kay-Gunnar Stubenrauch

Ralf Schumacher

Adelbert Grossmann

Yvo Graus

Frank Rebers

Martine Van Vugt

Josef Endl

Jan Olaf Stracke

Maria Elena Fuentes

Sandra Vereecken-Verploegen

Holger Klyszcz-Nasko

Peter Becker

Gerald Neubauer

Oliver Hailer

Lutz Helmrich

Maria Fuentes

Jan Stracke

Herein is reported a method for producing a polypeptide in monomeric form comprising the following step: recovering the polypeptide in monomeric form from an ion exchange chromatography material by applying a solution comprising a non-ionic polymer and an additive.

Ion exchange chromatography with improved selectivity for the separation of polypeptide monomers, aggregates and fragments by modulation of the mobile phase

Herein is reported a method for reducing the aggregation of an immunoglobulin in solution comprising the steps of i) comparing the amino acid sequence of the fourth framework region of the heavy chain of an antibody with a reference or germline sequence and determining whether one or more threonine residues and/or serine residues have been replaced by a different amino acid residue, and ii) modifying the amino acid sequence of the immunoglobulin by reverting the exchanged threonine residues and/or serine residues back to threonine or serine of the reference or germline sequence and thereby reducing the aggregation of an immunoglobulin in solution.

Sequence dependent aggregation

The instant specification discloses an antibody binding to IL-13R&#x3b1;1, inhibiting IL-13 bioactivity and comprising a variable heavy and a variable light chain, characterized in that the variable heavy chain amino acid sequence CDR3 of this antibody is selected from the group consisting of the heavy chain CDR3 sequences of SEQ ID NO: 1, 3, 5, 7 or 9, and this antibody is useful in the treatment of asthma and allergic diseases.

Antibodies against IL-13 receptor alpha1 and uses thereof

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Weilheim, Germany

Sebastian Neumann