USA as Represented by Secretary of the Navy

Sebastian A Diaz

Igor L Medintz

Kimihiro Susumu

Mario Ancona

Joyce A Breger

James N Vranish

Jesper Brask

Lipase activity can be detected with a biosensor that includes a quantum dot adhered to a construct having a lipase-cleavable ester to attach a fluorophore acceptor configured as a F&#xf6;rster resonance energy transfer (FRET) acceptor to the QD when the construct is bound thereto. Cleavage of the ester by a lipase results in a measurable reduction in FRET. In further embodiments, the cleavable ester can be used to detect esterase activity, or the ester could be replaced with a glycosidic linkage to detect glycoside activity.

Nanoparticle-based lipase biosensor utilizing a custom-synthesized peptidyl-ester substrate

A nanoparticle (for example, quantum dot) serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes.

Nanoparticle-attached enzyme cascades for accelerated multistep biocatalysis

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Washington, DC, United States of America

Sebastian A Diaz