United States of America As Represented by the Secretary of the Air Force

USA As Represented by Secretary of the Navy

Sean W Harshman

Anthony V Qualley

Rhonda L Pitsch

Kraig Strayer

Amanda Bowman

Jeffrey B Phillips

Brian A Geier

Darrin K Ott

Claude C Grigsby

Michael Charles Brothers

Jennifer A Martin

Claude C Gigsby

Michael Brothers

Rhonda Pitsch

Nicole M Schaeublin

Mark Hawkins

A flow regulation device for volatile and semi-volatile sampling onto adsorbent media is provided. The flow regulation device includes a housing having an inlet, an outlet, a secondary inlet that is open to the ambient, a real time flow meter, a controller in electronic communication with the flow meter, and a three-way valve. Gas is drawn through the adsorbent media, into the inlet, and then to the outlet by a vacuum source joined to the outlet. The controller is configured to: (1) actuate the three-way valve to put the vacuum source in-line with the inlet; (2) continuously monitor the flow of gas through the inlet and calculate the time necessary to sample a pre-set volume of gas; and (3) after the pre-set volume of gas passes through the inlet, actuate the three-way valve to put the vacuum source in-line with the secondary inlet.

Breath sample device including flow regulation device for volatile and semi-volatile sampling

Systems and methods for continuous real-time sweat sampling and analysis are disclosed. A sweat collection article and method of collecting sweat from a person's skin using the sweat collection article are provided. The sweat collection article includes: a sweat collecting tube placed adjacent to the person's skin, and at least one piece of flexible material that has an adhesive that adheres the article to the person's skin and is positioned to overlie one end of the tube. The second end of the sweat collecting tube is in fluid communication with an instrument such as a mass spectrometer that analyzes the sweat on a real-time basis. The systems and methods may further include a device for removing salt from the sweat that is arranged so that the sweat is transported to the device prior to being transported to the instrument for analyzing the sweat.

Systems and methods for real-time sweat sampling and analysis

A method of detecting hypoxia. Detecting hypoxia includes detecting, in exhaled breath, at least one indicator for hypoxia. The at least one indicator is selected from the group consisting of pentanal, 2-pentanone, 2-hexanone, 2-heptanone, 2-cyclopenten-1-one, and 4-butyrolactone.

Exhaled breath hypoxia biomarkers

Systems and methods for continuous real-time sweat sampling and analysis are disclosed. In one case, a sweat collection device and method of using the same are provided. The sweat collecting device has a main body with a sweat-collecting surface that is placed adjacent the person's skin. A sweat collecting tube is provided. The first end of the sweat collecting tube is joined to a bore in the main body of the device and the second end extends outward therefrom. In another case, a sweat collection article and method of collecting sweat from a person's skin using the sweat collection article are provided. The sweat collection article includes: a sweat collecting tube placed adjacent to the person's skin, and at least one piece of flexible material that has an adhesive that adheres the article to the person's skin and is positioned to overlie one end of the tube. In both cases, the second end of the sweat collecting tube is in fluid communication with an instrument such as a mass spectrometer that analyzes the sweat on a real-time basis. The systems and methods may further include a device for removing salt from the sweat that is arranged so that the sweat is transported to the device prior to being transported to the instrument for analyzing the sweat.

A biomarker diagnostic system includes a sensor to collect a sweat sample from a biological subject; a processor operatively connected to the sensor, wherein the processor is configured to perform metabolic and proteomic profiling of biomarkers in the sweat sample. The metabolic and proteomic profile is compared to a predetermined profile of the biomarkers and to determine a physiological status of the biomarkers. The system further includes a feedback unit operatively coupled to the sensor and the processor and configured to output physiological performance data based on the physiological status.

Identification, quantitation and analysis of unique biomarkers in sweat

Growing community of inventors

Fairborn, OH, United States of America

Sean W Harshman