Altius Institute for Biomedical Sciences

Sean Thomas

John A Stamatoyannopoulos

Fyodor Urnov

Jocelynn Pearl

Provided herein are polypeptides, compositions comprising the polypeptides and methods for genome editing and gene regulation (e.g., activation and/or repression) using the polypeptides or the compositions comprising the polypeptides, such as, DNA binding domains derived from the genus of. Also disclosed are DNA binding proteins that include a fragment of N-cap sequence of a TALE protein, such as, aTALE protein. Also disclosed are DNA binding proteins that include a fragment of N-cap sequence of a DNA binding protein derived from bacteria of the genus

Nucleic acid binding domains and methods of use thereof

DNA cleaving enzymes are disclosed. The DNA cleaving enzymes are fused to a heterologous DNA binding domain that is designed to bind to a target nucleic acid sequence. Nucleic acids and expression cassettes encoding the DNA cleaving enzymes are also provided. Methods for genome editing using the DNA cleaving enzymes, fusion proteins, and compositions thereof are disclosed herein.

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Seattle, WA, United States of America

Sean Thomas