University of California

Zymergen Inc.

Sean Poust

Jay D Keasling

Leonard Katz

Sara Da Luz Areosa Cleto

Andrew Ronald Hagen

Vytas SunSpiral

Kenneth S Bruno

Jeffrey L Fortman

Sergey Zotchev

Brian Chaikind

Arthur Muir Fong, Iii

Patrick J Westfall

Jingwei Zhang

Edyta Szewczyk

Hassan Abdulla

Paolo Boccazzi

Dylan Vaughan

Kyle Rothschild-Mancinelli

Jennifer Fredlund

Philip Weyman

William Serber

Matthew Jonathan Myers

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.

Hybrid polyketide synthases

A HTP genomic engineering platform for improving filamentous fungal cells that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols is provided. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. Methods for isolating clonal populations derived from individual fungal spores are also provided.

HTP genomic engineering platform for improving fungal strains

HTP genomic engineering platform for improving filamentous fungal cells that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. Methods can be carried out within optofluidic devices.

Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.

Liquid based selection and cell isolation

The present invention provides for a polyketide synthase (PKS) capable of synthesizing a carboxylic acid, said PKS comprising a synthetic or hybrid module. The present invention also provides for a host cell comprising the PKS and when cultured produces the carboxylic acid. In some embodiments, the carboxylic acid is adipic acid.

Producing adipic acid and related compounds using hybrid polyketide synthases

Growing community of inventors

El Cerrito, CA, United States of America

Sean Poust