Dow Agrosciences LLC

Ms-technologies Gmbh

Corteva Agriscience LLC

Sean Michael Russell

Terry R Wright

Bruce Marvin Held

Vaithilingam Sekar

Yunxing Cory Cui

Ning Zhou

Thomas Hoffman

Stephen N Novak

Sandra Grace Toledo

Dawn M Parkhurst

Julissa Colon

Justin Michael Lira

Thomas Meade

Timothy Denver Hey

Joel Jay Sheets

Jon Christopher Mitchell

Patricia C Apel-Birkhold

Kristin J Fencil

Robin Leola Thompson

Ze-Sheng Li

Donald J Merlo

Kenneth Edwin Narva

Manju Gupta

Steven Robert Webb

Aaron Todd Woosley

Andrew E Robinson

Ignacio Mario Larrinua

Joseph F Petolino

Jyoti R Rout

Dayakar Reddy Pareddy

Diane M Retallack

Nicole Arnold

Matthew J Henry

Kelley Ann Smith

Gregory Alan Bradfisch

Nathan Bard

Liu Shen

Sivarama Reddy Chennareddy

Lauren Clark

Yang Wang

Kelly Ann Smith

Tom Meade

Thom Hoffman

Kelley A Smith

Nicole L Arnold

Liu Yin Shen

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be 'stacked' with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TAQMAN® PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be 'stacked' with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified inA3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

Selectable marker genes

This invention relates to soybean event pDAB8264.42.32.1 and includes novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be 'stacked' with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TAQMAN PCR assays for the detection of Event pDAB8264.42.32.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof

A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

Excision of transgenes in genetically modified organisms

strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of thechromosome, the Ti plasmid, and plant transformation binary vectors, and uses for thesestrains are provided. Additionally,strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of thechromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included arestrains that harbor transformation-enhancing genes integrated into thechromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of thecells, and uses for thesestrains. Plants made using thesestrains are also described.

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This invention relates to soybean event pDAB8291.45.36.2, which includes a novel expression cassette comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding, and herbicide tolerant plants. In some embodiments, the event sequence can be 'stacked' with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to detection methods, including endpoint TaqMan PCR assays, for the detection of Event pDAB8291.45.36.2 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

Stacked herbicide tolerance event 8291.45.36.2, related transgenic soybean lines, and detection thereof

Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter fromorbidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.

A nucleic acid molecule encoding a 5-enolpyruvyl-3-phosphoshikimic acid synthase (EPSPS) that provides resistance to glyphosate is provided, that has been optimized for expression in both monocotyledonous and dicotyledonous plants and preferably in soybeans. Methods of use are also provided.

Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells

The subject invention provides novel amino acid sequences (including a consensus sequence) of the Avian Influenza A virus hemagglutinin protein. These newly constructed genes are designed to provide a broader spectrum of activity across the serotype family thus providing a basis for a vaccine that has broad heterologous disease protection. The novel genes have been further improved by the addition of strategic glycosylation sites into the amino acids sequences that they encode. These genes can also, optionally, be codon optimized for plant expression, inserted into the appropriate vector and cloned into plants for expression. Polypeptides produced by recombinant host cells or transgenic plants can also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals. Additionally, transgenic plant material may also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals.

DNA sequences, vectors and proteins of avian influenza hemagglutinin

The invention relates to isolated genes encodingtoxin complex (TC) proteins, and transgenic plants and host cells expressingtoxin complex (TC) proteins and having resistance to insects.

Genes encoding toxin complex proteins and uses thereof

Growing community of inventors

Carmel, IN, United States of America

Sean Michael Russell