Illumina Corporation

United States of America As Represented by the Secretary of the Air Force

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Sean M Ramirez

Joseph M Mabry

Alexandre Richez

Timothy S Haddad

Edwin Li

M Shane Bowen

Andrew Antony Brown

Wayne N George

Dajun Yuan

Rigo Pantoja

Ludovic Vincent

Audrey Rose Zak

Wei Wei

Anmiv Prabhu

Raymond Campos

David Louis Heiner

John M Beierle

James M Tsay

Sang Ryul Park

Kevan Samiee

Peyton Shieh

Gregory R Yandek

Tyler Jamison Dill

Scott William Bailey

Robert Yang

Yuxiang Huang

Naiqian Zhan

Valerie Uzzell

Brian M Moore

Kristina Munoz

Leonid Malevanchik

Samantha Schmitt

Sunmin Ahn

Allen E Eckhardt

Li Liu

Min-Jui Richard Shen

Hai Quang Tran

Shannon Kaplan

Bala Murali Venkatesan

Lewis J Kraft

Brian Douglas Mather

Xavier Von Hatten

Kathryn M Stephens

Justin Fullerton

Alex So

Hong Xie

Petr Capek

Julia Morrison

Steven Modiano

Gianluca Andrea Artioli

Timothy J Wilson

Anne Jager

Hayden Black

Maxwell Zimmerley

Neeraj Salathia

Krystal Sly

Innsu Daniel Kim

Sojeong Moon

Ryan Sanford

Michelle Higgins

Timothy Wilson

An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.

Arrays including a resin film and a patterned polymer layer

An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

Nucleic acid capture, concentration, and purification

A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

Flow cell package and method for making the same

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

A method of forming a hydrophobic and oleophobic surface. The method including spin coating an F-POSS with 3,3-dichloro-1,1,1,2,2,-pentafluoropropane/1,3-dichloro-1,1,2,2,3-pentafluoropropane onto an inert surface. The F-POSS has a structure:  Each Rrepresents a nonreactive, fluorinated organic group, Rrepresents a first monovalent organic group comprising at least two carbon atoms, and Rrepresents hydrogen or a second monovalent organic group comprising at least two carbon atoms. The F-POSS with 3,3-dichloro-1,1,1,2,2,-pentafluoropropane/1,3-dichloro-1,1,2,2,3-pentafluoropropane are then dried on the inert surface.

Synthesis of functional fluorinated polyhedral oligomeric silsesquioxane ('F-POSS')

Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

Spatial mapping of nucleic acid sequence information

Embodiments of present application are directed to microfluidic devices and particularly digital micro-plastic fluidic devices that are specifically designed to prevent sample contamination during sample processing, methods of manufacturing the same, and methods to improve sample analysis process by preventing sample contamination.

Cationic polymers and method of surface application

A method of manufacturing a fluorinated polyhedral oligomeric silsesquioxane. The method for manufacture includes opening a single edge of a closed-cage F-POSS and bridging the opened, single edge with a sulfate group. The sulfate group is converted to a disilanol, which is then reacted with a functional dichlorosilane having an organic functional group comprising at least three carbon atoms.

A polymer comprising polymerized units of norbornene fluoroalkyl polyhedral oligomeric silsesquioxane. According to aspects of the present invention, the polymer may be norbornene fluoroalkyl polyhedral oligomeric silsesquioxane. Still other aspects of the present invention may include a polymerized unit of an alkene chain derived from a cyclic alkene.

Controlled polymerization of functional fluorinated polyhedral oligomeric silsesquioxane monomers

A method of synthesizing peripherally asymmetric aryl polyhedral oligomeric silsesquioxane (ArPOSS) compounds. The method comprises: wherein Ph is phenyl and wherein R is 1-napthyl, 2-naphthyl, 9-anthracenyl, 9-phenanthrenyl, 1-pryenyl, and mixtures thereof.

Synthesis and applications of peripherally asymmetric aryl POSS compounds

A functional fluorinated polyhedral oligomeric silsesquioxane ('F-POSS'). The F-POSS, has a chemical structure: where Rrepresents a nonreactive organic group and at least one of Rand Rrepresents a chain comprising at least three carbon atoms.

Synthesis of functional fluorinated polyhedral oligomeric silsesquioxane (F-POSS)

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San Diego, CA, United States of America

Sean M Ramirez