Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Sean M Bertain

Kevin E McBride

Jack Q Wilkinson

Anthony John Kinney

Michael W Lassner

Linda A Castle

Steven Henry Bass

Theodore Mitchell Klein

Jun-Zhi Wei

Steven Gutteridge

Daniel Siehl

Henrik Albert

Jun-zhi Wei

Ajith Anand

Hyeon-Je Cho

Kay Walter

York Moy

John Lawrence Andreassi, Ii

Barbara Ann Marie Rosen

Kevin E Mcbride

Barabara Ann Marie Rosen

Methods and compositions for-mediated transformation of plants are provided. Methods include but are not limited to using anstrain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include anstrain, transfer DNAs, constructs and/or plasmids. These includestrains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.

Ochrobactrum-mediated transformation of plants

Compositions and methods comprising polynucleotides and polypeptides having ALS activity and tolerance to at least one ALS inhibitor are provided. In specific embodiments, the sequence has an increased preference for 2-ketobutyrate, when compared to an appropriate control, such as for example, HRA, and/or a preference for 2-ketobutyrate similar to a native ALS. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the ALS inhibitor tolerant sequences. Various methods of employing the ALS inhibitor tolerant sequences are provided. Such methods include methods for producing an ALS inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

Methods and compositions involving ALS variants with native substrate preference

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and fungal termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element. and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Recombinant expression cassettes with a fungal 3' termination sequence that function in plants

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop condon, and the fungal termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Recombinant expression cassettes with fungal 3' termination sequence that function in plants

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal 3&#x2032; termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

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Oakland, CA, United States of America

Sean M Bertain