Amyris, Inc.

Corn Products Development, Inc.

Sean Lund

Gale Wichmann

Chantal Garcia De Gonzalo

Shaina Jackson

Wenzong Li

Yi Xiong

Hailley Warbington

Svetlana Alekseyevna Borisova

Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of UGT76G1. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of rebaudioside M.

UDP-glycosyltransferase variants and uses thereof

Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding akaurenoic acid hydroxylase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides

Growing community of inventors

Emeryville, CA, United States of America

Sean Lund