Mirus Bio Corporation

Schering Aktiengesellschaft

Mirus Corporation

Roche Madison Inc.

Displaytech, Inc.

Arrowhead Madison, Inc.

Sean D Monahan

Jon Asher Wolff

James E Hagstrom

Vladimir G Budker

David B Rozema

Paul Michael Slattum

Vladimir S Trubetskoy

Wei Xu

Howard P Ng

Michael M Morrissey

Brad Owen Buckman

Meina Liang

John G Bauman

Imadul Islam

Guo Ping Wei

Richard Horuk

Joseph E Hesselgesser

Karen B May

Ameen F Ghannam

Wei Zheng

Zuchun Zhao

Kenneth Jay Shaw

Darren H Wakefield

Lori Higgs

Hans Herweijer

David L Lewis

Michael D Wand

So Wong

Aaron G Loomis

Alexander V Sokoloff

James Ludtke

Magdolna G Sebestyén

David M Walba

William N Thurmes

Rohini T Vohra

Julia Hegge

Kundalika M More

Lisa J Hanson

Jason Klein

Kirk Ekena

Paul Slattum

Vladimir M Subbotin

Larry F Whitesell

Matthew R Wolff

Lisa Nader

The present invention is directed to compounds, compositions, and methods useful for delivering polynucleotides or other cell-impermeable molecules to mammalian cells. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery vehicles. The use of multiple reversible linkages connecting component parts provides for physiologically responsive activity modulation.

Polyconjugates for in vivo delivery of polynucleotides

The present invention is directed to membrane active polymers useful for cellular delivery of compounds. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery conjugates. The use of reversible modification provides for physiologically responsive activity modulation.

Endosomolytic poly(acrylate) polymers

Endosomolytic poly(vinyl ether) polymers

The present invention is directed to a class of membrane active polymers useful for cellular delivery of compounds. Conjugation of the described membrane active polymers to targeting, anti-opsonization, and anti-aggregation agents provides polymers suitable for in vivo delivery. The use of multiple reversible linkages connecting component parts provides for physiologically responsive activity modulation.

Membrane active heteropolymers

Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.

Compositions and processes using siRNA, amphipathic compounds and polycations

Disclosed is a system for providing in vivo delivery of molecules or complexes to extravascular mammalian cells using an intravascular administration route. The molecules or complexes are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate transiently increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the molecule to the cell.

Delivery of molecules and complexes to mammalian cells in vivo

We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.

Compounds for targeting hepatocytes

Cyclic amidinium containing compounds and their methods of preparation are described. Compositions containing these compounds facilitate delivery of biologically active polymers to cells in vitro and in vivo.

Compositions for delivering nucleic acids to cells

A method of forming polymers in the presence of nucleic acid using template polymerization. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.

Process of making a compound by forming a polymer from a template drug

Compositions and methods for delivery of proteins and peptides to mammalian cells in vitro are described. Specifically, polypeptide-surfactant complexes formed from noncovalent hydrophobation of polypeptides and reversible hydrophobic modification of polypeptides are described. The compositions can be used to delivery positively charged, negatively charged and charge neutral polypeptides to cells.

Protein and peptide delivery to mammalian cells in vitro

An ionic polymer is utilized in 'recharging' (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.

Charge reversal of polyion complexes

This invention is directed to acyl piperazine derivatives which are useful as anti-inflammatory agents. This invention is also directed to pharmaceutical compositions containing the compounds of the invention, and methods of using the compounds to treat inflammatory disorders in humans.

Piperazine derivatives and their use as anti-inflammatory agents

A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.

Compositions and methods for drug delivery using pH sensitive molecules

Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.

Intravascular delivery of non-viral nucleic acid

Growing community of inventors

Madison, WI, United States of America

Sean D Monahan