Panagene Inc.

Invitros Co., Ltd.

Se Ryun Kim

Goon Ho Joe

Heekyung Park

Hoon Sung Jeh

Jae Hoon Oh

Sung-Kee Kim

Su Nam Kim

Chwang Siek Park

Chang Ju Park

Yongtae Kim

Sang Hun Lee

A gene amplification module is disclosed. According to one aspect of the present invention, provided is a gene amplification module including: a heat block in which a reaction container accommodation space is defined; a heating part provided below the heat block; a cooling part provided below the heating part; and a fluid supply part provided to face one side of the heat block, the fluid supply part being configured to supply a cooling fluid to the heat block, wherein sidewalls defining a circumference of the heat block are provided on the heat block, and one or more supply holes configured to supply the cooling fluid are defined in the sidewalls.

Gene amplification module

Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.

Melting curve analysis using PNA probe, method and kit for analyzing nucleotide polymorphism using melting curve analysis

Growing community of inventors

Daejeon, South Korea

Se Ryun Kim