Integrated Dna Technologies, Inc.

Scott Rose

Mark Aaron Behlke

Joseph Alan Walder

Joseph R Dobosy

Richard Owczarzy

Shawn Allen

Kristin Beltz

Andrei Laikhter

Yawfui Yong

Lingyan Huang

Stephen Gunstream

Derek M Thomas

Michael R Marvin

Susan M Rupp

Jeffrey A Manthey

Yong You

John Froehlig, Jr

Katherine Perschbacher

Steven A Henck

Joesph Dobosy

Andrey Tataurov

Joseph Dobosy

Joseph A Walder

Mark A Behlke

The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from(P.a.),(T.kod), andorganisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.

RNASE H2 mutants that reduce primer dimers and off-target amplification in RHPCR-based amplicon sequencing with high-fidelity DNA polymerases

Described herein are compositions and methods for the production and quantification of barcoded or unique molecular identifier (UMI)-labeled substrates. In one aspect, the substrate is a bead comprising a template oligonucleotide that is elongated by successive extension reactions to provide a bead with an oligonucleotide comprising a plurality of barcodes and conserved anchor regions. Methods are also described for quantifying the amount of template oligonucleotide loaded onto the substrate and the products of the extension reaction after each round and after the final extension.

Methods for production and quantification of unique molecular identifier-labeled beads

The invention provides methods for the synthesis of long oligonucleotides, genes and gene fragments. The methods include the manufacture of genes or gene fragments that can be then inserted into a variety of vectors.

Synthesis of long nucleic acid sequences

This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.

DNA polymerase mutants having enhanced template discrimination activity

Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mgcation and/or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.

RNase H-based assays utilizing modified RNA monomers

This invention relates to primers and probes for detecting Ebola virus and one or more subtypes of Ebola virus as well as kits including the probes and primers and methods of using the probes and primers.

Primers and probes for detection and discrimination of Ebola virus

The invention is directed to an in vitro method for joining a first set of double-stranded (ds) DNA molecules. Small molecules acting as chaperone agents are identified that promote efficient and rapid assembly (that is, joining) of overlapping double-stranded DNA fragments.

Efficient and rapid method for assembling and cloning double-stranded DNA fragments

The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3' end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.

The present invention provides methods of cleaving a nucleic acid strand to initiate, assist, monitor or perform biological assays.

A composition comprising an oligonucleotide having the structure 5'-Y-L-X-L-Y-3&#x2032;. Ycomprises a sequence of one or more DNA or RNA nucleotides, including a first nucleotide Nhaving a 3&#x2032; phosphate covalently linked to L. Ycomprises a sequence of one or more DNA or RNA nucleotides, including a second nucleotide Nhaving a 5&#x2032; phosphate covalently linked to L. Land Leach independently are a direct bond or a C-Calkyl, alkynyl, alkenyl, heteroalkyl, substituted alkyl, aryl, heteroaryl, substituted aryl, cycloalkyl, alkylaryl, or alkoxyl group. X is Ris a hydrogen or a C-Calkyl. M is a label or ligand comprising a fused polycyclic aromatic moiety.

Methods for enhancing nucleic acid hybridization

The present invention provides methods that more accurately predict melting temperatures for duplex oligomers. The invented methods predict the Tof chimeric duplexes containing various amounts of locked nucleic acid modifications in oligonucleotide strands.

Methods for predicting stability and melting temperatures of nucleic acid duplexes

The invention provides novel anthraquinone compositions that are useful as broad-spectrum quenchers of fluorescence and provides methods for making and using them. The anthraquinone quenchers can be conjugated to a variety of biologically relevant compounds, including lipids, nucleic acids, polypeptides, and more specifically antigens, steroids, vitamins, drugs, haptens, metabolites, toxins, environmental pollutants, amino acids, peptides, proteins, nucleotides, oligonucleotides, polynucleotides, carbohydrates, and their analogs. The invention also provides kits comprising, in one or more containers, at least one anthraquinone quencher dye composition of the present invention, and instructions for using that composition.

Growing community of inventors

Coralville, IA, United States of America

Scott Rose