Agilent Technologies, Inc.

Hologic, Inc.

Scott Robert Happe

Joseph A Sorge

Andrew Firmin

Joseph Hoang Hai Ong

Emily Marine Leproust

Julia Barboza

Provided herein are various methods for enriching a target fragment that is present in randomly sheared genomic DNA. In some embodiments, the method may involve hybridizing randomly sheared genomic DNA to a halo probe to produce a first circular complex, and then enzymatically digesting the overhanging ends of the genomic fragment. Other embodiments may include hybridizing randomly sheared genomic DNA to an RNA oligonucleotide that comprises a region that hybridizes to a fragment of the randomly sheared genomic DNA to produce an RNA/DNA duplex. The overhanging ends of the genomic fragment in the RNA/DNA duplex can then be enzymatically digested.

Target enrichment of randomly sheared genomic DNA fragments

A method for determining an efficiency of target enrichment from a DNA library, includes: adding a negative control sequence and a positive control sequence to the DNA library, or picking a negative control sequence and/or a positive control sequence from the library; determining a pre-capture amount of the negative control sequence and a pre-capture amount of the positive control sequence; performing enrichment of a target sequence from the DNA library using a bait sequence to produce a post-capture library; determining a post-capture amount of the negative control sequence and a post-capture amount of the positive control sequence in the post-capture library; and determining the efficiency of the target enrichment, based on the post-capture amount of the positive control sequence, the post-capture amount of the negative control sequence, the pre-capture amount of the positive control sequence, and the pre-capture amount of the negative control sequence.

Quantitative PCR-based method to predict the efficiency of target enrichment for next-generation sequencing using repetitive DNA elements (lines/sines) as negative controls

The invention provides methods, compositions and kits for generating a signal indicative of the presence of a target nucleic acid sequence in a sample comprising forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with upstream and downstream oligonucleotides, and cleaving the cleavage structure with a nuclease to generate a signal. The presence of a detectable signal is indicative of the presence of a target nucleic acid sequence and a non-invasive cleavage structure.

Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction

The invention relates to compositions and methods for generating a signal indicative of the presence of a target nucleic acid in a sample utilizing a primer-probe duplex.

Growing community of inventors

Austin, TX, United States of America

Scott Robert Happe