University of Washington

Twinstrand Biosciences, Inc.

Scott R Kennedy

Jesse J Salk

Michael Hipp

Rosa Ana Risques

Daniela Nachmanson

Elizabeth Schmidt

The present technology relates generally to methods and compositions for targeted nucleic acid sequence enrichment, as well as uses of such enrichment for error-corrected nucleic acid sequencing applications. In some embodiments, highly accurate, error corrected and massively parallel sequencing of nucleic acid material is possible using a combination of uniquely labeled strands in a double-stranded nucleic acid complex in such a way that each strand can be informatically related to its complementary strand, but also distinguished from it following sequencing of each strand or an amplified product derived therefrom. In various embodiments, this information can be used for the purpose of error correction of the determined sequence.

Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing

Methods for making and using reagents and adapters for use in nucleic acid sequencing applications are disclosed herein. In several embodiments, adapters and reagents and the methods of making such adapters and reagents can be used for Duplex Sequencing applications.

Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters

Growing community of inventors

Seattle, WA, United States of America

Scott R Kennedy