University of Utah Research Foundation

Canon U.s.a., Inc.

Canon U.S. Life Sciences, Inc.

Canon Virginia, Inc.

Scott O Sundberg

Kenton C Hasson

Ivor T Knight

Chris Felice

Kazuhiko Hasegawa

Ronald Kurz

Ralph McCann

Jason Clemons

Brian P Murphy

Eric D Schneider

Carl Thomas Wittwer

Bruce Kent Gale

Johnathan Stuart Coursey

Alex K Flamm

Robert Andrew Palais

Weidong Cao

Sami Kanderian

Takayoshi Hanagata

Jeanette Yang Paek

Makoto Ogusu

Renee Howell

David Li

Fernando Weber DeCastro

Yoichi Murakami

Christina Pysher

Robert J Pryor

Chris J Felice

Joseph T Myrick

Carl T Wittner

Fernando DeCastro

Robert A Palais

Some embodiments of a micro-fluidic device include at least one inlet hole located on an inlet side of the microfluidic device, the inlet hole consisting of a plurality of holes with diameters smaller in size than a diameter of the at least one inlet hole, at least one outlet hole located on an outlet side of the microfluidic device opposite the inlet side; and a micro-channel, where the plurality of holes are connected to the micro-channel.

Multi hole inlet structure

A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50&#xb0; C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.

High speed nucleic acid melting analysis

The present invention relates to the use of one or more amplicons as temperature calibrators. In some embodiments, the calibrators may be used to calibrate the temperature of a microfluidic channel in which amplification and/or melt analysis is performed. In some embodiments, the amplicons may be genomic, ultra conserved elements and/or synthetic. The amplicon(s) may have a known or expected melt temperature(s). The calibrators may be added to primers of study or may follow or lead the primers of study in the channel. The amplicon(s) may be amplified and melted, and the temperature(s) at which the amplicon(s) melted may be determined. The measured temperature(s) may be compared to the known temperature(s) at which the amplicon(s) was expected to melt. The difference(s) between the measured and expected temperatures may be used to calibrate/adjust one or more temperature control elements used to control and/or detect the temperature of the channel.

Thermal calibration

Cleaning station assembly

Cartridge assembly

A microfluidic flow cell having a body with a fluid transport channel disposed therein, the fluid transport channel having a proximal end and a distal end defining a fluid flow path, a fluid inlet port disposed at the proximal end of the fluid transport channel at a central portion of the body and an outlet port disposed at the distal end of the fluid transport channel at an outer portion of the body, and a plurality sample wells disposed in the fluid transport channel substantially perpendicular to the fluid flow path in the fluid transport channel. The microfluidic flow cell may have hundreds or thousands of individual, sub-microliter sample wells. The microfluidic flow cell can be filled by applying a flowable liquid to the inlet port and spinning the flow cell to cause fluid to flow into fluid transport channel. The microfluidic flow cells described herein can be used in a variety of applications where small sample size and/or a large number of replicates are desirable.

Microfluidic flow cell

Growing community of inventors

Yorktown, VA, United States of America

Scott O Sundberg