Ixsys, Incorporated

Scott M Glaser

William D Huse

William P MacConnell

The invention provides a Vitaxin antibody and a LM609 grafted antibody exhibiting selective binding affinity to &agr; &bgr; . The Vitaxin antibody consists of at least one Vitaxin heavy chain polypeptide and at least one Vitaxin light chain polypeptide or functional fragments thereof. Also provided are the Vitaxin heavy and light chain polypeptides and functional fragments. The LM609 grafted antibody consists of at least one CDR grafted heavy chain polypeptide and at least one CDR grafted light chain polypeptide or functional fragment thereof. Nucleic acids encoding Vitaxin and LM609 grafted heavy and light chains as well as nucleic acids encoding the parental non-human antibody LM609 are additionally provided. Functional fragments of such encoding nucleic acids are similarly provided.

Anti-&agr;v&bgr;3 recombinant human antibodies, nucleic acids encoding same

A high throughput device is provided for simultaneously isolating periplasmic fractions from multiple samples of host cells. The device can be formatted to operate in a series. A method for simultaneously isolating multiple periplasmic fractions is also provided. The method includes (a) providing a high throughput device for isolating periplasmic fractions from multiple samples of host cells having an outer membrane via attaching a removable sample chamber having a membrane chamber wall to a vacuum chamber and applying a vacuum thereto (b) removing media with the vacuum and (c) removing the outer membrane of the host cells (d) attaching a collection chamber to the vacuum chamber and applying a vacuum thereto in order to carry out a collection of the periplasmic fractions that pass through the membrane of the chamber wall from the host cells. A workstation is also provided which includes one or more high throughput devices for simultaneously isolating periplasmic space fractions from a plurality of samples of host cells, and a device for simultaneously culturing a plurality of samples. preferably the membrane of the chamber is a neutral nylon membrane having an average pore diameter of,to,micron.

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Seattle, WA, United States of America

Scott M Glaser