Illumina Corporation

Scott L Butler

Mark S Chee

Jian-Bing Fan

Arnold Oliphant

John R Stuelpnagel

Min-Jui Richard Shen

Kenneth M Kuhn

John E Stuelpnagel

Mun-Jui Richard Shen

A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

Multiplex nucleic acid reactions

The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.

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Sandwich, United Kingdom

Scott L Butler