Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Other

Scott E Nichols

Chun Ping Li

Kanwarpal Singh Dhugga

Peizhong Zheng

George William Singletary

Matthias Liebergesell

Jian G Dong

William Dean Hitz

Kristen Briggs

Court A Saunders

William James Gordon-Kamm

Anthony John Kinney

Bo Shen

Keith S Lowe

Oliver P Peoples

Guihua Lu

Mitchell Christian Tarczynski

Kevin L Stecca

Gan-Yuan Zhong

Wesley B Bruce

Rudolf Jung

Lynne Eileen Sims

Derrick Ryan Witcher

Larry R Beach

Changjiang Li

Odd-Arne Olsen

Evgueni Ananiev

Darren B Gruis

Patricia Lynne Fallis

Stein E Lid

Jennifer A Lorentzen

Cunxi Wang

Patrica Lynne Fallis

Kimberly F Glassman

Francedillaois J Loiselle

Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.

Recombinant constructs and their use in reducing gene expression

The invention relates to the genes encoding 3-oxoacyl-[acyl carrier protein(ACP)] reductase (OAR). Compositions and methods for producing polyhydroxyalkanoate in transformed plants and transformed host cells are provided. Such methods find use in producing biodegradable thermoplastics in host cells and plants. Isolated nucleotide molecules, expression cassettes, isolated polypeptides and genetically manipulated host cells, plants, plant tissues, plant cells and seeds are also provided.

OAR polynucleotides, polypeptides and their use in PHA production in plants

The invention relates to the genetic manipulation of plants to produce polyhydroxyalkanoate, particularly in the peroxisomes. Methods for producing such polymers in plants and host cells are provided. Such methods find use in producing biodegradable thermoplastics in plants and other organisms. Nucleotide molecules, expression cassettes, and genetically manipulated host cell, plants, plant tissues, and seeds are also provided.

Production of polyhydroxyalkanoate in plants

Methods are provided for altering grain characteristics by introducing into plants, isolated nucleic acid molecules that can be used to produce transgenic plants characterized by altered number, type, or configuration of aleurone cells within the seed. Also provided are isolated nucleic acids that encode maize dekproteins, vectors capable of expressing such nucleic acid molecules, host cells containing such vectors, and polypeptides encoded by such nucleic acids.

Methods for improving seed characteristics

The invention relates to the genetic manipulation of plants to produce polyhydroxyalkanoate, particularly in the peroxisomes. Methods for producing such polymers in plants and host cells are provided. Such methods find use in producing biodegradable thermoplastics in plants and other organisms. Nucleotide molecules, expression cassettes, and genetically manipulated host cells, plants, plant tissues, and seeds are also provided.

Methods are provided that diminish UDPGdH expression in plant cells as compared to wild-type plant cells. These methods find use in increasing the nutritional value of animal feed and improving processes for extraction of starch from plant seeds. Also provided are expression vectors capable of diminishing UDPGdH expression in plant cells, and transgenic plants expressing diminished levels of UDPGdH.

Plant uridine diphosphate-glucose dehydrogenase genes, proteins, and uses thereof

The invention relates to the genetic manipulation of plants to increase oil accumulation in plant tissues, particularly seeds. Methods for decreasing &#x3b2;-oxidation in plants and optimizing oil accumulation in a seeds are provided. The methods find use in increasing the accumulation of oil or particular oil constituents in plant seeds. Isolated nucleotide molecules, isolated proteins, expression cassettes and transformed plants, plant tissues and plant cells are additionally provided.

Methods for regulating beta-oxidation in plants

The invention relates to the genes encoding 3-oxoacyl-&lsqb;acyl carrier protein(ACP)&rsqb; reductase (OAR). Compositions and methods for producing polyhydroxyalkanoate in transformed plants and transformed host cells are provided. Such methods find use in producing biodegradable thermoplastics in host cells and plants. Isolated nucleotide molecules, expression cassettes, isolated polypeptides and genetically manipulated host cells, plants, plant tissues, plant cells and seeds are also provided.

The invention relates to genes encoding polyhydroxyalkanoate synthases. Compositions and methods for producing polyhydroxyalkanoate are provided. Such compositions and methods find use in producing biodegradable thermoplastics in host cells and transgenic plants. Isolated nucleotide molecules, isolated polypeptides, expression cassettes and genetically manipulated host cells, plants, plant tissues, plant cells and seeds are also provided.

Polyhydroxyalkanoate synthase genes

The present invention provides methods of making paper, utilizing glucans, produced by the glucosyltransferase B, C or D enzyme of the species instead of modified starches. The present glucans are functionally similar to currently utilized modified starches and are particularly useful in the coating step of paper manufacture. The present glucans also exhibit thermoplastic properties and impart gloss to the paper during the coating step.

Glucan-containing compositions and paper

Isolated nucleic acid molecules are provided that encode maize UDPGdH variant UDPGdH, and mutant UDPGdH proteins. These nucleic acid molecules can be used to produce transgenic plants having altered quality or quantity of starch. Also provided are vectors capable of expressing such nucleic acid molecules, host cells containing, such vectors, and polypeptides encoded by such nucleic acids.

The present invention provides methods of making paper, utilizing glucans, produced by the glucosyltransferase B, C or D enzyme of the species,instead of modified starches. The present glucans are functionally similar to currently utilized modified starches and are particularly useful in the coating step of paper manufacture. The present glucans also exhibit thermoplastic properties and impart gloss to the paper during the coating step.

Substitutes for modified starch and latexes in paper manufacture

Compositions providing increased energy content of animal feed using plant crop biomass which include a polyhydroxyalkanoate have been developed. In one embodiment, the compositions can be prepared using conventional techniques for harvesting and processing plant crops into forms useful as animal feed, wherein the plant, or parts thereof, have accumulated PHA, preferably in excess of 2% by dry weight of the plant tissue. In a preferred embodiment, the PHA is accumulated in corn or an oilseed. The feed compositions can include the PHA-containing meal byproduct from corn or oilseed processing. In another embodiment, the PHA can be provided with the green tissue of plants, such as clover, alfalfa, sorghum, and silage corn.

Animal nutrition compositions

The invention provides isolated Rgp nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering RGP levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

Growing community of inventors

West Chester, PA, United States of America

Scott E Nichols