California Institute of Technology

University of Southern California

University of California

Research Corporation Technologies, Inc.

Other

Eth Zurich

Clinical Micro Sensors, Inc.

Osmetech Technology, Inc.

Clinical Micro Systems, Inc.

Scott E Fraser

Thomas J Meade

Jon Faiz Kayyem

Russell E Jacobs

Thai Viet Truong

Daniel M Schwartz

Periklis Pantazis

Axel Scherer

Michael Roukes

Sameer Walavalkar

Andrea Martin Armani

Jerry E Solomon

Richard C Flagan

Michael C Cross

Robert H Grubbs

Changjun Yu

Niles A Pierce

Jost G Vielmetter

Bruce D Irvine

Robert H Terbrueggen

Gary Fife Blackburn

Chieh-feng Chang

Tsu-Te Judith Su

Robert Dirks

Morteza Gharib

Mark E Davis

Kerry J Vahala

Demetri Psaltis

Stephen D O'Connor

Andrew Peter Homyk

John H Hong

Gregory H Bearman

Akinleye C Alabi

William M Jones

Ye Pu

Brandon C Marin

Julia A Kornfield

Xiquan Cui

Yu Hsuan Chen

Sameer Sudhir Walavalkar

Jeffrey P Fingler

Francesco Cutrale

Thomas W Welch

Stephen E Creager

William P Dempsey

David S Koos

John M Choi

Pu Wang

Russell D Lansford

Sara Madaan

Jelena Culic-Viskota

Willy Supatto

Justin P Gallivan

Daniel B Holland

Julian Michael Tyszka

Peter H Siegel

Andrea R Tao

Jessica L Arlett

Cavour Yeh

Joseph P Dunham

Sotirios Masmanidis

Sean A Mendoza

Mary Dickinson

Timothy Wells Hunt

Tao Lu

Jason Junge

Ilene Sugino

Matthew S Mattson

Rajan P Kulkarni

Eun Sang Koo

Wen Shi

Stephen J O'Connor

Wenhong Li

Kevin A Mendoza

Kevin Keomanee-Dizon

Nicholas T Rinella

Lada Georgieva

Chang Jun Yu

Simon Restrepo

Matthew J Allen

Fred Shimabukuro

Michael Liebling

Arian Farouhar

Yun Kee

Andrey V Demyanenko

Hsiao-Ju Chiang

Systems and methods are provided for increasing photon collection during imaging with a light-sheet fluorescence microscope. In one example, one or more adaptive optical elements may be positioned in a detection light path between a detection objective and an imaging sensor. A depth of field of the detection objective is adjusted as a function of a thickness of an excitation light-sheet used to illuminate a sample during imaging. As a result, the detection objective captures more fluorescence photons generated by light-sheet excitation.

Optimized photon collection for light-sheet microscopy

Systems and methods are provided for multi-spectral or hyper-spectral fluorescence imaging. In one example, a spectral encoding device may be positioned in a detection light path between a detection objective and an imaging sensor of a microscope. In one example, the spectral encoding device includes a first dichroic mirror having a sine transmittance profile and a second dichroic mirror having a cosine transmittance profile. In addition to collecting transmitted light, reflected light from each dichroic mirror is collected and used for total intensity normalization and image analysis.

High throughput snapshot spectral encoding device for fluorescence spectral microscopy

This invention relates to a hyperspectral imaging system for denoising and/or color unmixing multiple overlapping spectra in a low signal-to-noise regime with a fast analysis time. This system may carry out Hyper-Spectral Phasors (HySP) calculations to effectively analyze hyper-spectral time-lapse data. For example, this system may carry out Hyper-Spectral Phasors (HySP) calculations to effectively analyze five-dimensional (5D) hyper-spectral time-lapse data. Advantages of this imaging system may include: (a) fast computational speed, (b) the ease of phasor analysis, and (c) a denoising algorithm to obtain the minimally-acceptable signal-to-noise ratio (SNR). An unmixed color image of a target may be generated. These images may be used in diagnosis of a health condition, which may enhance a patient's clinical outcome and evolution of the patient's health.

Hyperspectral imaging system

Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

Optimized gene editing utilizing a recombinant endonuclease system

Described herein is a method to create dendritic biocompatible polymers from pairs of complementary dendritic nucleic acid monomers in a controlled manner, using polymerization triggers. The dendritic monomers are constituted of nucleic acids and an organic polymer capable of self-assembly. Each polymer contains approximately 200 dendrites that can be used to attach labels and constitute a biologically compatible signal amplification technology. Depending on the context this technology could be used to reveal the presence of a large variety of analytes such as specific nucleic acid molecules, small molecules, proteins, and peptides.

Method to generate biocompatible dendritic polymers for analyte detection with multimodal labeling and signal amplification

Volumetric imaging with selective volume illumination (SVI) using light field detection is provided using various systems and techniques. A volumetric imaging apparatus includes a light source configured to emit an illumination light that propagates via an illumination light path to illuminate a three-dimensional (3D) sample; and an optical system arranged with respect to the light source to receive a light field, which comes from the illuminated 3D sample. The light field propagates via a detection light path, and the light source, the optical system, or both, are configurable to perform SVI, which selects a volume of a 3D-confined illumination of the 3D sample based on the 3D sample to be illuminated and a light field detection (LFD) process to be applied. Further, the volume of the 3D-confined illumination is a selected 3D volume of the 3D sample to be particularly excited by the 3D-confined illumination for imaging.

Optimized volumetric imaging with selective volume illumination and light field detection

Methods for fabricating flexible substrate nanostructured devices are disclosed. The nanostructures comprise nano-pillars and metallic bulbs or nano-apertures. The nanostructures can be functionalized to detect biological entities. The flexible substrates can be rolled into cylindrical tubes for detection of fluidic samples.

Fabrication and self-aligned local functionalization of nanocups and various plasmonic nanostructures on flexible substrates for implantable and sensing applications

Volumetric imaging with selective volume illumination (SVI) using light field detection is provided using various systems and techniques. A volumetric imaging apparatus includes a light source configured to emit an illumination light that propagates via an illumination light path to illuminate a three-dimensional (3D) sample; and an optical system arranged with respect to the light source to receive a light field, which comes from the illuminated 3D sample. The light field propagates via a detection light path, and the light source, the optical system, or both; are configurable to perform SVI, which selects a volume of a 3D-confined illumination of the 3D sample based on the 3D sample to be illuminated and a light field detection (LFD) process to be applied. Further, the volume of the 3D-confined illumination is a selected 3D volume of the 3D sample to be particularly excited by the 3D-confined illumination for imaging.

Implementations of the present disclosure include methods, systems, and computer-readable storage mediums for image reconstruction. Actions include receiving an image acquired by an endoscopic system including an optical fiber bundle with multiple optical fibers, each optical fiber being surrounded by cladding, determining in the image fiber core locations corresponding to the optical fibers, reconstructing missing information from the image using interpolation performed in accordance with the fiber core locations, the missing information corresponding to artifacts in the acquired image that result from the cladding, and providing a fiber-pattern removed image, in which the artifacts in the acquired image are removed using the missing information.

Fiber pattern removal and image reconstruction endoscopic devices and related methods

This disclosure relates to the field of Optical Coherence Tomography (OCT). This disclosure particularly relates to an OCT system that generates an image with improved quality. In one example, the OCT system may generate an improved Bscan image by using multiple shaping functions to shape the raw A-scans. In another example, the OCT system may generate the improved B-scan image by forming multiple apodization patterns on a detector and acquiring raw A-scans by using the apodization patterns. A better diagnosis of a health condition may be reached by using the improved images generated by the OCT system of this disclosure.

Optical coherence tomography (OCT) system with improved image quality

This invention relates to a hyperspectral imaging system for denoising and/or color unmixing multiple overlapping spectra in a low signal-to-noise regime with a fast analysis time. This system may be configured to carry out Hyper-Spectral Phasors (HySP) calculations to effectively analyze hyper-spectral time-lapse data. For example, this system may be configured to carry out Hyper-Spectral Phasors (HySP) calculations to effectively analyze five-dimensional (5D) hyper-spectral time-lapse data. Advantages of this imaging system may include: (a) fast computational speed, (b) the ease of phasor analysis, and (c) a denoising algorithm to obtain the minimally-acceptable signal-to-noise ratio (SNR). An unmixed color image of a target may be generated. These images may be used in diagnosis of a health condition, which may enhance a patient's clinical outcome and evolution of the patient's health.

Devices, systems, and methods for detection of an analyte in a sample are disclosed. In some embodiments, an optical sensor can include a metallic layer and a plurality of dielectric pillars extending through the metallic layer. A plurality of regions of concentrated light can be supported in proximity to the ends of the plurality of dielectric pillars when a surface of the metallic layer is illuminated. Concentrated light within one or more of these regions can interact with an analyte molecule, allowing for detection of the analyte.

Optical sensor for analyte detection

A sclera or contact lens with an image of an eye that is fenestrated, or uniformly speckled with many sub-millimeter sized transparent regions or holes, is disclosed. The micro fenestrated contact lens can be worn on a strabismic, or misaligned, eye so that its image is aligned with the wearer's dominant eye. The fenestrations allow the wearer to see through the opaque or translucent image printed on the contact lens, thus allowing binocular vision even though his or her strabismic eye is covered.

Micro fenestrated contact lens

The invention relates to a method and a system to achieve spatially (e.g. three-dimensionally) confined photomodulation at the focal volume () in a ample () mounted in a microscope system, comprising two or more laser light sources () emitting light () of different wavelengths adapted to excite a material in an identical number of independent excitation steps to a higher vibrational state from which the material relaxes, either emitting a conversion light to be detected ('photoexcitation') or modulating the spectral properties of the material ('photomodulation').

Method and device to achieve spatially confined photointeraction at the focal volume of a microscope

Methods and systems for nanopillar sensors are described. Nanopillars can be defined on a substrate, and metal deposited on the nanopillars. A thermal treatment can reflow the metal on the nanopillars forming metallic bulbs on the top end of the nanopillars. These structures can have enhanced optical detection when functionalized with biological agents, or can detect gases, particles and liquids through interaction with the metal layer on the nanopillars.

Reflowed gold nanostructures for surface enhanced raman spectroscopy

Growing community of inventors

La Canada, CA, United States of America

Scott E Fraser