Battelle Memorial Institute

National Technology & Engineering Solutions of Sandia, LLC

Other

University of California

Natl Tech & Engineering Solutions of Sandia, LLC

Scott E Baker

John Michael Gladden

Ziyu Dai

Jon K Magnuson

Joonhoon Kim

Jed J Lynn

Morgann C Reilly

Blake A Simmons

Saori Amaike Campen

Jinxiang Zhang

Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.

Gene targets for improved enzyme production in fungi

The present invention provides for anhost cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.

Promoter useful for high expression of a heterologous gene of interest in

Fungi, such as, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described arefungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.

[object Object]

A portable adjustable headrest that is releasably secured to the seatback of a casual seating. The headrest generally includes a headrest support bar, and a pair of rosettes that attach to a pair of vertical arms of the headrest support bar for vertical adjustment. First and second clamp bars having telescoping extensions for receipt in channels defined in each of the rosettes. Each clamp bar further includes a hook member opposite the telescoping extension, that define a slot for receipt of the chair's seatback frame. The clamp bars can each be selectively positioned in order to horizontally adjust the width of the headrest such that the clamp bars, as a whole is extensible and retractable to suit the needs of both wider designed chairs and more narrowly designed chairs. A cushioned member having a pocket sized for receiving an upper portion of the support bar, and further including an exterior surface and interior foam layer.

Portable adjustable headrest

Provided herein are fungi, such as, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.

Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression

Growing community of inventors

Richland, WA, United States of America

Scott E Baker