Syngenta Participations Ag

Ab Enzymes Gmbh

Scott Betts

Michael B Lanahan

Shib Sankar Basu

Dale Wayne Skalla

Koen Hendrickx

Andrea Nelson

Stephanie Winslow

Makoto Ono

Sandra Lynn Voltrath

This invention relates to methods of the detection of enzymatically active enzymes in feed added in the pre-pelleting stage. Further, this invention relates to the field of detecting thermotolerant enzymes added to feed in the pre-pelleting stage. In particular, the invention relates to the detection of a phytase enzyme, in particular anphytase or anderived phytase in feed, such as Quantum&#x2122; Phytase.

Extraction methods and assays for feed enzymes

The invention provides a synthetic phytase polynucleotide which is optimized for expression in plants and which encodes at thermotolerant phytase, as well as isolated thermotolerant phytase enzyme. Also provided are feed or food products comprising a thermotolerant phytase, and transgenic plants which express the thermotolerant phytase. Further provided are methods for making and using thermotolerant phytases, e.g., a method of using a thermotolerant phytase in feed and food processing.

Thermotolerant phytase for animal feed

Provided is a nucleotide sequence of a promoter from a gene encoding a 55 kDa maize prolamin family protein. Also provided are methods that utilize the 55 kDa maize prolamin family gene promoter to express heterologous sequences in plants, expression cassettes that include the 55 kDa maize prolamin family gene promoter, and plants transformed with expression cassettes that include the 55 kDa maize prolamin family gene promoter.

Promoter from maize prolamin seed storage protein and uses thereof

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Research Triangle Park, NC, United States of America

Scott Betts