Columbia University

Scott A Kanner

Gordana Vunjak-Novakovic

John O'Neill

Donald O Freytes

Matthew D Bacchetta

Henry M Colecraft

Gopal Singh

Joshua Sonett

The present disclosure provides, inter alia, a recombinant engineered deubiquitinase (DUB) and methods for treating or ameliorating an inherited ion channelopathy, such as long QT syndrome, Brugada syndrome, or cystic fibrosis, in a subject. Further provided are methods for screening mutations causing such inherited ion channelopathies for a trafficking-deficient mutation that is treatable by the recombinant engineered DUB disclosed herein.

Compositions and methods for using engineered deubiquitinases for probing ubiquitin-dependent cellular processes

The vasculature of a donor lung is perfused with a lung preserving fluid to preserve its structure. At the same time, a decellularization fluid is perfused through the airways, which strips away donor cells. The decellularized region is then seeded with pulmonary cells of the transplant recipient, which regenerate the lung. The pulmonary cells may be derived from stem cells, and the decellularization can be targeted to reduce the quantity of cells required.

Functional recovery of human lungs for transplantation

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Brooklyn, NY, United States of America

Scott A Kanner