E.i. Dupont De Nemours and Company

Pioneer Hi-Bred International, Inc.

Corteva Agriscience LLC

Other

E. I. Du Pont Nemours and Co.

Saverio Carl Falco

Zhongsen Li

Stephen M Allen

J Antoni Rafalski

Rebecca E Cahoon

Andrew Mark Cigan

Michael W Lassner

Huirong Gao

Sergei Svitashev

Zhan-Bin Liu

Anthony John Kinney

Omolayo O Famodu

Christopher Jay Scelonge

Narendra S Yadav

James Jefferson Smith

Derek Jantz

Janet Ann Rice

Joshua K Young

Zude Weng

Layo O Famodu

John R Bedbrook

Roy S Chaleff

Barbara J Mazur

Hajime Sakai

Lu Liu

Mitchell Christian Tarczynski

Emil M Orozco, Jr

James J English

Ericka Bermudez

Azalea S Ong

Sharon Jo Keeler

Zhan-bin Liu

Catherine J Thorpe

Christopher R Somerville

William Dean Hitz

Perry G Caimi

Karlene H Butler

Guo-Hua Miao

Kevin L Stecca

Leszek Aleksander Lyznik

Jian-Ming Lee

Carl A Maxwell

Maria Restrepo-Hartwig

Jinrui Shi

Andrew Cigan

Quinn Qun Zhu

Carl R Simmons

Keith S Lowe

Edgar Benjamin Cahoon

David J Peterson

Dennis L Bidney

Yiwen Fang

Peizhong Zheng

Feng Han

Joan Tellefsen Odell

Sabine Susanne Hantke

Susan Knowlton

Michele Morgante

Xiping Niu

Anthony D Guida, Jr

Lisa L Huang

James S Schwaber

Jan Antoni Rafalski

Shawn L Anderson

Karin N Lohman

Lynn Marie Abell

Michael Ramaker

Mike Lassner

L Aleksander Lyznik

Michael Lassnser

Mary Elizabeth Locke

Chok-Fun Chan Chui

Cristopher R Somerville

Anthony Dominick Guida

Sabine Ursula Epelbaum

Raymond Ervin Mcdevitt

Mitchell Tarczynaski

Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.

Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker, and compositions thereof

Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

Plant genome modification using guide RNA/Cas endonuclease systems and methods of use

Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

Methods for producing a complex transgenic trait locus

Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

Methods and compositions for generating complex trait loci

Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.

Compositions and methods comprising sequences having meganuclease activity

Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.

Methods and compositions for targeted integration in a plant

Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.

Methods and compositions for producing male sterile plants

Growing community of inventors

Wilmington, DE, United States of America

Saverio Carl Falco