Illumina Corporation

Illumina Singapore Pte. Ltd.

Illumina Cambridge Limited

Agency for Science, Technology and Research

National University of Singapore

Saurabh Nirantar

Sergio Peisajovich

Misha Golynskiy

Seth McDonald

Kay Klausing

Hamed Tabatabaei Ghomi

Michael Previte

Molly Min He

Matthew William Kellinger

Maybelle Darlene Kho Go

Wen Shan Yew

Alexandra Exner

Jasmine Puay Suan Chua

Eric Brustad

Eric Murtfeldt

John Farid Ghadessy

Mariko Matsuura

Ryan Craig

Kyrie Johnson

Disclosed herein include methods of generating a single stranded deoxyribonucleic acid (ssDNA) scaffold comprising nucleotides with modified bases using a recombinant terminal deoxynucleotidyl transferase (TdT). The recombinant TdT can comprise an amino acid sequence that is at least 80% identical to aTdT, or a fragment thereof, and for example comprise one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His420 in theTdT.

Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase

Disclosed herein include recombinant terminal deoxynucleotidyl transferases (TdTs). In some embodiments, the recombinant TdT comprises an amino acid sequence that is at least 80% identical to a bovine TdT, wherein the recombinant TdT comprises one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His421 in the bovine TdT.

Thermostable terminal deoxynucleotidyl transferase

Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

Polymerases, compositions, and methods of use

Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.

The invention relates to methods of identifying a binding partner of a target molecule within a plurality of analyte molecules, including a plurality of peptides and/or proteins. The target molecule is physically combined with a target labeling nucleic acid molecule. Each member of the plurality of analyte molecules is physically linked to an analyte labeling nucleic acid molecule, each analyte labeling nucleic acid molecule comprising a selected nucleotide sequence. This specific nucleotide sequence may include a sequence encoding a peptide/protein combined therewith. The target molecule is contacted with the analyte molecules and a complex between the target molecule and an analyte molecule forms. The mixture is subdivided into compartments. The target labeling nucleic acid molecule and the analyte labeling nucleic acid molecule are linked and the plurality of compartments allowed to disintegrate. The linked nucleic acid molecule is retrieved and the sequence determined.

Methods of identifying a pair of binding partners

Growing community of inventors

Singapore, Singapore

Saurabh Nirantar