New England Biolabs, Inc.

Saulius Vainauskas

Christopher H Taron

Siu-Hong Chan

Theodore B Davis

Romualdas Vaisvila

Heidi Erika Johnson

Xiaofeng Shi

The present disclosure relates, according to some embodiments, to compositions, methods, and/or kits for producing vaccinia capping enzyme. For example, active, heterodimers of vaccinia capping enzyme may be produced as fusions comprising D1 and D12 subunits. Vaccinia capping enzyme fusion proteins may further comprise a linker.

Vaccinia capping enzyme compositions and methods

Provided herein is an &#x3b1;-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The &#x3b1;-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the &#x3b1;-fucosidase are provided, as well as a method for analyzing glycoproteins. The &#x3b1;-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.

Cleavage of fucose in N-glycans

Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases and/or oxygenases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.

Methods and compositions for discrimination between cytosine and modifications thereof, and for methylome analysis

Methods and compositions for discrimination between cytosine and modifications thereof and for methylome analysis

Growing community of inventors

Newburyport, MA, United States of America

Saulius Vainauskas