Avecia Biotechnology, Inc.

Satya Kuchimanchi

Nanda Dulal Sinha

Saied Shaikh

A process for the preparation of a poly(alkoxylated) oligonucleotide is provided. The process comprises reacting an oligonucleotide which has been purified by ultrafiltration with a poly(alkoxide) thereby to form a poly(alkoxylated) oligonucleotide. The poly(alkoxyalted) oligonucleotide may be separated from non-poly(alkoxyalted) oligonucleotide by ultrafiltration under denaturing conditions, such as the presence of organic solvents, for example, ethanol; the presence of urea; the addition of chaotropic salts, for example perchlorate and guanidinium salts; the presence of formamide; and the application of heat, for example a temperature of up to about 70&#xb0; C.

Process for the preparation of poly(alkoxylated) oligonucleotides

Nitrogen-protecting groups are removed from the exocyclic nucleobase portion of a 2'-O protected nucleotide or 2&#x2032;-halo nucleotide by contacting the nucleotide with an inorganic base. Typical is the removal of t-butylphenoxyacetyl protecting groups from the nucleobase portion of a 2&#x2032;-O protected nucleotide on which the 2&#x2032;-O protecting group is t-butyldimethylsilyl, removal or deprotection being accomplished by contact with a potassium carbonate solution.

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Milford, MA, United States of America

Satya Kuchimanchi