Fuji Yakuhin Kogyo Kabushiki Kaisha

Satoshi Miyamoto

Kazushi Iwata

Akira Oshima

Yasuo Bai

Yasuteru Muragaki

Eisaku Matsumoto

A method for determining human type III, IV and VI collagen peptides by way of enzyme immunoassay according to the sandwich technique using a monoclonal antibody and a polyclonal antibody each to the human type III, IV or VI collagen peptide, characterized in that the monoclonal antibody to the collagen peptide is used as an antibody to be coated onto a solid phase and/or an antibody to be labeled with an enzyme. Also disclosed is a sandwich technique using two different monoclonal antibodies. This method can be carried out with a smaller amount of samples in a simple manner to obtain a precise result and is thus useful for diagnosis of hepatic diseases.

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Wakayama, Japan

Satoshi Miyamoto