Tanabe Seiyaku Company, Limited

Daicel Chemical Industries, Ltd.

Satoshi Minobe

Taizo Watanabe

Tetsuya Tosa

Tadashi Sato

Ichiro Chibata

Takeji Shibatani

Masahiro Kagotani

Zenjiro Honda

Shinji Nagamatsu

Makoto Masuda

Tomonobu Ase

Takashi Nishimura

There is disclosed a method for determining an endotoxin by reacting the endotoxin adsorbed on an adsorbent having specific endotoxin adsorbability with an endotoxin detecting reagent, including the steps of:

Method for determining endotoxin and apparatus therefor

A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent composed of a nitrogen-containing heterocyclic compound bonded to a water-insoluble carrier directly or through a spacer, and after or without eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by the Limulus method.

Method for determining pyrogen content

Pyrogens are removed out of a solution thereof by (1) treating the solution with a membrane and then (2) treating the resulting solution with a pyrogen adsorbent. The obtained solution is useful in pharmaceutical fields.

Process for removing pyrogens

A method for removing a pyrogen from a solution of a pyrogen-containing substance such as an amino acid, a nucleic acid base, an antibiotic, a hormone, a vitamin, an enzyme, an antibody or the like comprising contacting the solution with an adsorbent to adsorb the pyrogen, which is characterized in that the adsorbent comprises a water-insoluble carrier and a nitrogen-containing heterocyclic compound of the formula:

Method for reducing the pyrogen content of or removing pyrogens from

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Otsu, Japan

Satoshi Minobe