Gekkeikan Sake Company Limited

Takeda Chemical Industries, Inc.

Satoshi Imayasu

Eiji Ichikawa

Hideaki Yamada

Yasuhiro Sumino

Shigeya Kakimoto

Yoji Hata

Hiroki Ishida

Yasuhisa Abe

Hiromoto Hisada

Tetsuyoshi Suizu

Koji Suginami

Akitsugu Kawato

According to the protein expression system in which a variety of desired useful proteins are highly produced by fusing a coding region of useful protein gene to the downstream of a promoter region of a tyrosinase-encoding gene (melO) of by a usual method of DNA manipulation, transferring a plasmid containing the resulting novel fusion gene into and incubating the thus-obtained transformant, various proteins can efficiently be produced at a high purity and in a high yield.

High expression system of proteins

Alcoholic liquors containing carbamide are treated with the urease which has the optimal pH for the activity in an acid region, especially in the region of pH2 to 5. By such treatment, the carbamide can be completely decomposed and removed from the alcoholic liquors in a much smaller amount of urease and in a short time as compared with a conventional treatment according to a urease having the optimal pH in the neutral to alkaline region. This method is more advantageous from the viewpoint of practical use and the quality of the thus obtained alcoholic liquors are superior to that obtained by the conventional treatment.

Quality improvement of alcoholic liquors

Growing community of inventors

Kyoto, Japan

Satoshi Imayasu