Nikon Corporation

Satoshi Ikeda

Naoki Fukutake

Shota Tsuchida

A microscope device comprises an illumination optical system for illuminating a sample, a detection optical system for receiving light from the sample, a detector for detecting the light from the sample via the detection optical system and outputting a detection signal of the light, a data processor for generating at least one of a three-dimensional refractive index distribution and a two-dimensional phase distribution in the sample based on the detection signal of the light output from the detector, and a modulation element group that is provided at a position of a pupil or a position conjugate with the pupil in only the illumination optical system, and has light transmittance changing continuously within a surface of the pupil or within a surface conjugate with the pupil.

Microscope device and data generation method using microscope

A quantitative phase image generating method for a microscope, includes: irradiating an object with illumination light; disposing a focal point of an objective lens at each of a plurality of positions that are mutually separated by gaps &#x394;z along an optical axis of the objective lens, and detecting light from the object; generating sets of light intensity distribution data corresponding to each of the plurality of positions based upon the detected light; and generating a quantitative phase image based upon the light intensity distribution data; wherein the gap &#x394;z is set based upon setting information of the microscope.

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Yokohama, Japan

Satoshi Ikeda