Arkray, Inc.

Kyoto Daiichi Kagaku Co., Ltd.

Satoshi Fukunaga

Kyouichi Ohshiro

Atsuko Nanbu

Harumi Uenoyama

Masato Nakayama

Hironori Hasegawa

Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently.

Method for detecting prozone phenomenon, analysis method, device for detecting prozone phenomenon, and analysis device

A concentration measuring method includes selecting a calibration curve optimum for computing the concentration of a measurement target substance from a plurality of calibration curves based on an output from a reaction system containing the target substance and a reactant capable of reacting with the target substance, and computing the concentration of the target substance based on the optimum calibration curve and the output. Each of the calibration curves is prepared based on a plurality of outputs generated upon lapse of a same reaction time from a plurality of standard reaction systems each containing a standard reagent of a known different concentration and the reactant. The plurality of calibration curves differ from each other in reaction time based on which the calibration curves are prepared.

Concentration measuring method

A method for measuring the concentration or the activity of UTI quickly and easily at high sensitivity. A urine sample, a buffer solution, a trypsin solution and a substrate solution are mixed and the trypsin activity is then measured. Thus, the UTI concentration in the urine sample is determined. In this case, a substrate solution having only L-BAPNA is used as the substrate, and the surfactant is mixed in at least one selected from the buffer solution and the enzyme solution. The mixing ratio of the surfactant is about 1 wt. % in the entire enzyme reaction solution. Examples of the surfactant include polyoxyethylene (40) octylphenylether, polyoxyethylene (10) octylphenylether, 3-[(3-cholamido propyl)dimethylammonio]-propanesulfonic acid, 3-[(3-cholamido propyl)dimethylammonio]-2-hydroxypropanesulfonic acid, and polyoxyethylene sorbitan monolaurate. As shown in FIG. 1, the sensitivity improves when using L-BAPNA.

Method for measuring the concentration or the activity of protease

This invention provides a method for measuring the concentration of urinary trypsin inhibitors which is excellent in precision and reproducibility, whose operation is simple, and in which a possibility of damaging a plastic cell is eliminated. The method for measuring the concentration of urinary trypsin inhibitors comprises mixing an urine sample, a protease solution containing trypsin, and a buffer solution, adding a substrate solution to the mixture to cause the enzyme reaction, and measuring the activity of the enzyme, wherein the buffer solution is prepared so that it contains at least 0.15 .mu.mol calcium per 1 .mu.g of the trypsin but no more than 100 .mu.mol calcium per 1 ml of the urine sample in the reaction mixture, and wherein the substrate solution is prepared by dissolving the substrate in an organic solvent and diluting the mixture solution with aqueous medium, wherein at least one of an amphoteric surfactant and a nonionic surfactant is added to at least one of the organic solvent and the aqueous medium.

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Kyoto, Japan

Satoshi Fukunaga