Nisshinbo Industries, Inc.

Other

Satoshi Aotsuka

Osamu Hasegawa

Soichiro Takenishi

Hirofumi Uchimiya

Takahisa Hayashi

Yuri Ihara

DNA is produced by preparing oligomers having partial sequences selected according to a specific scheme based on a target nucleotide sequence, and performing PCR using two of single strand DNAs base-paired at their 3' ends as primers and templates to prepare DNA, in a specific manner.

Method for producing DNA

Nucleotide sequences of cotton plant cDNA clones randomly selected are determined. Homology search is performed between the sequences and known gene sequences registered in the data base. Thus a gene, which controls the fiber formation mechanism in cotton plant and which can be used for industrially useful improvement, is isolated from clones having homology to a gene in the data base.

Cotton plant gene

mRNA was extracted at the stage for cotton plant fibrous cells to accumulate cellulose, and cDNA's complementary thereto were synthesized to construct a cDNA library. Clones of a number of 750 were arbitrarily selected from the library, and they were randomly subjected to sequencing. Those having homology to an amino acid sequence deduced from a gene of cellulose 4-.beta.-glucosyltransferase (bcsA) of cellulose synthase operon of acetic acid bacterium were selected from obtained nucleotide sequences of the respective clones. Thus, DNA coding for cellulose synthase was obtained.

Growing community of inventors

Chiba, Japan

Satoshi Aotsuka