Mitsubishi Denki Kabushiki Kaisha

Tosoh Corporation

Satoru Oonaka

Masanori Abe

Masashi Mori

Toshinori Hayashi

Juichi Saito

Masao Nakagawa

Masashi Oguchi

Atsumi Koyama

Daisuke Omoto

A method of detecting micrometastasis of tumor cells in a sample obtained from a region of a subject other than the primary focus of the tumor by using a first primer complementary to part of a specific sequence in the RNA from tumor cells and a second primer homologous to part of the specific sequence (either of which additionally has a promoter sequence for an RNA polymerase at the 5' end), which comprises (1) synthesizing a cDNA by the action of an enzyme having RNA-dependent DNA polymerase activity by using the specific sequence as a template, (2) degrading the RNA strand in the RNA-DNA double strand by an enzyme having a ribonuclease H activity (to give a single-stranded DNA), (3) forming a double-stranded DNA having a promoter sequence which can be transcribed into an RNA homologous or complementary to the specific sequence by using the single-stranded DNA as a template by the action of an enzyme having DNA-dependent DNA polymerase activity, and (4) transcribing the double-stranded DNA into an RNA transcript (which acts as a template in the subsequent cDNA synthesis in the reaction (1)) by the action of an enzyme having RNA polymerase activity and detecting the mRNA.

Method of detecting micrometastasis

A simultaneous broadcasting system, a transmitter, and a receiver therefor use a first frequency bandwidth for a wide area broadcasting and a second frequency bandwidth for a local area broadcasting obtained by dividing a frequency bandwidth of one broadcasting channel. In the simultaneous broadcasting system, a same program for the wide area broadcasting is transmitted based on an OFDM modulation method by using the first frequency bandwidth and a different program for each local area station is transmitted by using a different spreading code allocated for each local are station based on a SS modulation method.

Simultaneous broadcasting system, transmitter and receiver therefor

Growing community of inventors

Tokyo, Japan

Satoru Oonaka