Kyorin Pharmaceutical Co., Ltd.

National Institute of Advanced Industrial Science and Technology

Satoru Iwanami

Yasushi Kohno

Naoki Ando

Kazuhiko Kuriyama

Shinji Kudou

Hidenori Nagai

Takahiro Tanase

The invention provides a reciprocal-flow-type nucleic acid amplification method performing thermal cycling by reciprocating a sample liquid between a denaturation temperature zone and an elongation-annealing temperature zone with a connected microchannel including at least a curved channel corresponding to the denaturation temperature zone, a curved channel corresponding to the elongation-annealing temperature zone, a linear or curved intermediate channel that connects the aforementioned curved channels, and a connector to connect to a liquid delivery mechanism for enabling movement of the sample liquid. The method includes moving the sample liquid in the channel by the liquid delivery mechanism that is open to atmospheric pressure when liquid delivery is stopped, and measuring a fluorescence intensity for each thermal cycle at a predetermined point on the channel corresponding to the denaturation temperature zone and at a predetermined point on the channel corresponding to the elongation-annealing temperature zone to perform real-time PCR.

Nucleic acid amplification method

The present invention provides diaryl ether derivatives that exhibit significant immunosuppressive effects with less side effects. The diaryl derivatives of the present invention are represented by the following general formula (1):

Diaryl ether derivative, addition salt thereof, and immunosuppressant

The present invention provides diaryl sulfide derivatives that exhibit significant immunosuppressive effects with less side effects. The diaryl derivatives of the present invention are represented by the following general formula (1): One example is 2-amino-2-[4-(3-benzyloxyphenylthio)-2-chlorophenyl]propyl-1,3-propanediol.

Diaryl sulfide derivative, addition salt thereof, and immunosuppressant

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Ibaraki, Japan

Satoru Iwanami