Canon Kabushiki Kaisha

Satoru Hatakeyama

Hidenori Shiotsuka

Junta Yamamichi

Tsuyoshi Nomoto

Masaru Kaieda

Takeshi Imamura

Takashi Ikeda

Manabu Komatsu

Hideaki Okamoto

Hiroyuki Hashimoto

Norihiko Utsunomiya

It is intended to provide a target substance detection element wherein a target substance capturing body for capturing target substances is immobilized with good orientation in a desired region on the surface of the target substance detection element, a method for producing the target substance detection element, and a detection method using the target substance detection element. The present invention provides a target substance detection element for detecting the presence or absence or concentration of a target substance in a sample, characterized in that: the target substance detection element includes at least a detection substrate including plural layers and a target substance capturing body immobilized on the surface of the detection substrate; the target substance capturing body has at least a first peptide region specifically recognizing a first layer of the plural layers constituting the detection substrate and binding to the first layer and a second peptide region specifically recognizing a second layer different from the first layer of the plural layers and binding to the second layer; and the first layer and the second layer are adjacent to each other.

Target substance detection element, target substance detection method, and method for producing target substance detection element

The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens &#x3bc;m and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis.

Treatment solution, method for pretreatment, method for acquiring information and method for detection

A capturing molecule having an association containing a plurality of polypeptide chains that specifically bind to different sites of a target substance, characterized in that each of the polypeptide chains has a domain having a hypervariable loop structure at a binding site binding to the target substance and an association portion for forming the association, and the polypeptide chains are associated via the association portions present in the polypeptide chains.

Bispecific capturing molecule

A capturing molecule having not less than two domains specifically binding to different sites of a target substance, wherein the not less than two domains comprise (1) a first domain having a hypervariable loop structure at a binding site to the target substance, and (2) a second domain having no hypervariable loop structure at a binding site to the target substance.

Target substance capturing molecule

It is intended to provide an anti-photocrosslinking group antibody capable of specifically binding to a photocrosslinking group and available in the binding of a substrate and a substance of interest in a microstructure, a complex protein including at least the antibody or at least a portion thereof, and a technique for use thereof in the detection of a target substance. The present invention provides a protein having at least the structure of an antibody that recognizes a photocrosslinking group.

Protein, method for immobilizing protein, structure, biosensor, nucleic acid, vector and kit for detecting target substance

A device for introducing fine magnetic particles into a cell by applying an electric field to a reaction field where a sample solution containing cells and the fine magnetic particles are introduced, in order to form pores in the surface of the cell, and then applying a magnetic field to the reaction field to introduce the fine magnetic particles into the cell through the pore(s).

Growing community of inventors

Kawasaki, Japan

Satoru Hatakeyama