Takeda Chemical Industries, Inc.

Satoru Asahi

Muneharu Doi

Takahiko Yano

Yutaka Tsunemi

There is provided an economical, efficient and industrially useful method of producing vitamin B.sub.12 which comprises cultivating a microorganism belonging to Rhizobium cobalaminogenum FERM BP-4429 which is capable of producing vitamin B.sub.12 in a culture medium and recovering the vitamin B.sub.12. The microorganism is isolated from soil and has no formation of acids from L-arabinose. Further, there are no straight chain fatty acids 21:1 or 19:0.

Method of producing vitamin B12 using rhizobium cobalaminogenum ferm

There is provided an economical, efficient and industrially useful method of producing vitamin B.sub.12 which comprises cultivating a microorganism belonging to Rhizobium cobalaminogenum which is capable of producing vitamin B.sub.12 in a culture medium to produce vitamin B.sub.12 and recovering the produced vitamin B.sub.12. There are also provided a microorganism belonging to Rhizobium cobalaminogenum which is capable of producing vitamin B.sub.12 and a microorganism Rhizobium cobalaminogenum 27B74 (FERM BP-4429).

Culture of Rhizobium cobalaminogenum capable of producing vitamin

Uridine is produced by cultivating in a culture medium a uridine-producing microorganism, which belongs to the genus Bacillus and which is deficient in uridine nucleoside phosphorylase activity and is resistant to a pyrimidine analogue, and recovering the accumulated uridine. This method has the advantage of substantially avoiding the by-production of uracil and uridylic acid.

Production of uridine

Cytidine and/or deoxycytidine are produced at high yields by culturing cytidine deaminase activity-defective microbes of the genus Bacillus, which have resistance to pyrimidine analogs and with the ability to produce cytidine and/or deoxycytidine in a medium.

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Takatsuki, Japan

Satoru Asahi