Sumitomo Chemical Company, Limited

Satomi Nishikawa

Kenji Oeda

Fumiharu Ishige

Promoters capable of efficiently expressing a gene of interest in plants include (1) either a DNA containing SEQ ID No:1 or SEQ ID No:7, or a biological functional equivalent DNA containing SEQ ID No:1 or SEQ ID No:7 that have modifications in their respective nucleotide sequence provided that each retains more than 90% identity to the nucleotide sequence of any region containing at least 250 bp within SEQ ID No:1 or SEQ ID No:7, respectively. Terminators capable of efficiently expressing a gene of interest in plants include either a DNA containing SEQ ID No:2, or a biological functional equivalent DNA containing a SEQ ID No:2 that has a modified nucleotide sequence provided that it retains more than 90% identity to the nucleotide sequence of any region containing at least 250 bp within SEQ ID No:2.

Plant promoters and plant terminators

The present invention provides a promoter which is characterized in that it has following DNA sequence (a) or (b) and a DNA having the nucleotide sequence shown by SEQ ID:No.1, (a) a DNA sequence having the nucleotide sequence represented by at least nucleotide numbers 112-246 within the nucleotide sequence shown by SEQ ID:No.2, (b) a DNA sequence having the nucleotide sequence represented by at least base numbers 186-282 within the nucleotide sequence shown by SEQ ID:No.3 (hereinafter referred to as the present promoter), a chimera gene having the above promoter, a vector having the above promoter, a transformant characterized in that the promoter, the chimera gene or the vector is introduced into a host cell, a method for expressing gene comprising a step of expressing the desired gene in a host cell under the control of the promoter, use of the promoter for controlling the expression of a desired gene in a host cell, and a method for producing the promoter. According to the present invention, there becomes possible to provide a compact promoter suitable for more highly expressing the desired gene in the particular tissue than in other tissues in a host.

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Ashiya, Japan

Satomi Nishikawa