Meiji Seika Kaisha, Ltd.

Meiji Seika Pharma Co., Ltd.

Sato Aihara

Takeshi Murakami

Manabu Watanabe

Naomi Sumida

Koji Yanai

Kaoru Okakura

Kentaro Yamamoto

Koichiro Murashima

Naoki Midoh

Horst Kleinkauf

Hiroyuki Anzai

Koichi Miyamoto

Kouji Yanai

Tetsuya Yasutake

Takafumi Futamura

There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.

Nucleic acid construct comprising pyripyropene biosynthetic gene cluster and marker gene

The present invention provides an enzyme that synthesizes a cyclic depsipeptide, particularly the substance 1022, and a gene thereof. A cyclic depsipeptide synthetase according to the present invention comprises (a) an amino acid sequence of SEQ ID NO: 2 or (b) a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that have one or more modifications selected from a substitution, a deletion, an addition and an insertion and has cyclic depsipeptide synthetase activity. A cyclic depsipeptide synthetase gene according to the present invention comprises a nucleotide sequence encoding a cyclic depsipeptide synthetase. The present invention also provides a recombinant vector and a transformant for expressing the cyclic depsipeptide synthetase, and a mass production system for the cyclic depsipeptide. The present invention further provides a method for producing the cyclic depsipeptide synthetase.

Cyclic depsipeptide synthetase and method for recombinant production

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Odawara, Japan

Sato Aihara