Pacific Biosciences of California, Inc.

Sassan Sheikholeslami

Lei Sun

Natasha Popovich

Erik Miller

Satwik Kamtekar

Michael W Hunkapiller

Steven Lin

Jaime Juan Benitez-Marzan

Aparna Vedula

David Christopher Scherer

Yufeng Qian

Methods, compositions, and systems for distributing nucleic acids into array regions are provided. The methods, compositions, and systems utilize nucleic acid condensing agents to increase efficiency of distribution of the nucleic acids into the array regions. Various methods for facilitating distribution of the nucleic acids to the array regions are provided.

Loading nucleic acids onto substrates

The present disclosure provides improved methods for isolating asymmetrically-primed and/or asymmetrically-tagged nucleic acid complexes that find use in downstream analytical analyses, including sequence analysis. Compositions comprising such complexes and kits and systems for generating such complexes are also provided.

Methods and compositions for isolating asymmetric nucleic acid complexes

The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.

Methods and compositions for loading of polymerase complexes

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San Francisco, CA, United States of America

Sassan Sheikholeslami