Axon Instruments, Inc.

Sasha Dromaretsky

Yuri Vladimirovich Osipchuk

Stephen J Smith

Yuriy Roll

A fluorescent optical imaging system ( ) produces two separate spots (S and S ) on a sample ( ) by a pair of excitation laser beams (B and B ) that are generated by first and second lasers (L and L ). Excitation laser beams (B and B ) pass at slightly different angles, first through an aperture ( ) of a 45&deg; fold mirror ( ), and then through an objective element ( ). As a result, emission light beams ( ) are generated from each illuminated spot (S and S ) and are reflected and redirected by mirror ( ) through a secondary lens ( ) before reaching one of two detectors (PMT and PMT ). Emission beam ( ) reflects off a second 45&deg; mirror ( ) prior to reaching detector (PMT ), while emission beam ( ) travels directly to (PMT ). If desired, optical separation elements ( ), such as dichroic filters, prisms, or gratings, can be positioned in front of each detector (PMT and PMT ). Fluorescent optical imaging system ( ) may employ a scanning system ( ) for illuminating and imaging the entire area of sample ( ).

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Davis, CA, United States of America

Sasha Dromaretsky