Qiagen Gmbh

Qiagen Manchester Limited

Sascha Strauss

Holger Engel

Nicola Jo Thelwell

Andy Wende

Sabine Werner

Francesca Di Pasquale

Daniel Müller

Vanessa Siegmund

Kathrin Wolf

Francesca Dipasquale

The present invention relates to methods and kits for the specific detection of() serotypes O157:H7 and/or O145:H28. The methods and kits are based on the detection of newly identified sequence regions, which have a very high sequence identity betweenserotypes O157:H7 and O145:H28 and which are not present in any other knownserotype or bacteria. This sequence region thus allows for selective detection ofO157:1-17 and/or O145:H28 from other bacteria, especially otherserotypes. Furthermore the present invention shows that a 3 bp InDel sequence in O157:H7 allows for distinguishing between O157:H7 and O145:H28, which allows for selective detection of O157:H7 over O145:H28 and vice versa. Furthermore, the invention provides oligonucleotides useful for said detection.

Highly specific and sensitive methods for detecting enterohemorrhagicserotypes O157:H7 and/or O145:H28

Short tandem repeat (STR) markers are genetic elements that are frequently used in the fields of forensic analysis, paternity determination and detection of genetic diseases and cancers. Such analysis involves the amplification of STR loci. Technically, this can be challenging due to sequence variations in the flanking regions of the locus. In the case of SE33, previous amplification efforts have failed. The present invention describes a set of primers for the amplification of SE33 and a method for the analysis of the presence and/or level of SE33, also in combination with other STRs.

Method of amplification of a short tandem repeat locus

The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.

Method for quantifying human DNA using an internal control

The present invention relates to a method, kit and use of various nucleic acid sequences for deleting and/or quantifying one or more nucleic acids of a genome in a sample. Wherein the nucleic acid is amplified and the locus that is amplified is a multi copy locus within the genome, the multicopy locus has copies on at least two different chromosomes and the amplification product is detected and/or quantified.

Method for detecting and/or quantifying human DNA

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Solingen, Germany

Sascha Strauss