Roche Diagnostics Operations, Inc.

Hoffmann-la Roche Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Sascha Lutz

Christoph Boehm

Juergen Spinke

Thorsten Brueckner

Adelbert Grossmann

Norbert Oranth

Nadine Losleben

Thomas Dolbinow

Thomas Manuel Keller

Thomas Fischer

Gregor Ocvirk

Eloisa Lopez-Calle

Josef Roedl

Heinz Kontschieder

Jürgen Spinke

Wolfgang Burkhardt

Miriam Ruf

Pamela Espindola

Domenik Wensorra

Erik Beiersdorf

Sabrina Adler

Alexander Keuchel

Bruntje Esrom

Michael Herbel

Thorsten BRÜCKNER

Thomas Keller

Christoph BÖHM

Herbel Michael

A medical system for determining an analyte quantity in a blood sample via a cartridge that spins around a rotational axis. The cartridge may include: a separation chamber that separates blood plasma from the sample; a processing chamber containing a reagent with a specific binding partner which binds to the analyte to form an analyte specific binding partner complex; a first valve structure connecting the separation chamber to the processing chamber; a measurement structure to measure the quantity of the analyte, wherein the measurement structure includes a chromatographic membrane with an immobilized binding partner for direct or indirect binding of the analyte or the analyte specific binding partner complex, and an absorbent structure that is nearer to the axis than the membrane; a second valve structure connecting the processing chamber to the measurement structure; and a fluid chamber filled with a washing buffer and fluidically connected to the measurement structure.

Determining a quantity of an analyte in a blood sample

The present disclosure refers to a sensor assembly for an IVD analyzer, the sensor comprising two opposite substrates with at least one fluidic conduit for receiving a sample. The electrodes of different types of electrochemical sensors are arranged on the two opposite substrates facing the at least one fluidic conduit for coming in contact with the sample and determining sample parameters, wherein the counter electrodes and the reference electrodes are formed on one substrate and the working electrodes are formed on the opposite substrate. This achieves optimal sensor-working conditions in terms of a homogeneous and symmetrical electric field density and enables a sensor assembly with simpler geometry and smaller size.

Sensor assembly

A cartridge including a structural part with a hole surrounded at least partially by a recess, so that a wall is formed that separates the hole from the recess. The cartridge includes a liquid pack, wherein the liquid pack comprises a surrounding seal portion and a liquid containing portion. The liquid pack is arranged at least partially in the hole, in that a circumferential outer line of the seal portion protrudes over the hole.

Cartridge with liquid pack

A method of performing an optical measurement of an analyte in a processed biological sample using a cartridge is provided. The cartridge is operable for being spun around a rotational axis. The method comprises: placing the biological sample into a sample inlet; controlling the rotational rate of the cartridge to process a biological sample into the processed biological sample using a fluidic structure; controlling the rotational rate of the cartridge to allow the processed biological sample to flow from the measurement structure inlet to an absorbent structure via a chromatographic membrane, and performing an optical measurement of a detection zone on the chromatographic membrane with an optical instrument. An inlet air baffle reduces evaporation of the processed biological sample from the chromatographic membrane during rotation of the cartridge.

Measurement of an analyte with a cartridge

An automatic analyzer cartridge, spinnable about a rotational axis, has fluid and aliquoting chambers, a metering chamber connected to a vent that is nearer to the rotational axis than the metering chamber, first and second ducts connecting the fluid and aliquoting chambers, and the metering and aliquoting chambers, respectively. Metering chamber side walls taper away from a central region, wherein capillary action next to the walls is greater than in the central region. Fluid flows to the metering chamber using capillary action via the second duct that has an entrance and exit in the aliquoting and metering chambers, respectively; the exit being closer to the rotational axis than the entrance. A downstream fluidic element connects to the metering chamber via a valve. A fluidic structure receives and processes a biological sample into the processed biological sample and has a measurement structure that enables measurement of the processed biological sample.

Rotatable cartridge for processing and analyzing a biological sample and dispensing method therewith

A micro fluid filtration system () preferably for increasing the concentration of components contained in a fluid sample has a fluid circuitry (). The fluid circuitry () comprises the following elements: A tangential flow filtration element () capable for separating the fluid sample into a retentate stream and a permeate stream upon passage of the fluid, an element for pumping () for creating and driving a fluid flow through the fluid circuitry () and at least one element for obtaining information about the properties of the fluid sample within the circuitry. The circuitry further comprises a plurality of conduits () connecting the elements of the fluid circuitry () through which a fluid stream of the fluid sample is conducted. The circuitry () has a minimal working volume of at most 5 ml, which is the minimal fluid volume retained in the elements and the conduits () of the circuitry () such that the fluid can be recirculated in the circuitry () without pumping air through the circuitry (). An integrated microfluidic element () of the circuitry () contains the functionality of at least two elements of the group of elements of the circuitry ().

Micro flow filtration system and integrated microfluidic element

A method and cartridge for determining an amount of at least two analytes in a biological sample and an automatic analyzer are disclosed. The cartridge may comprise a cartridge inlet, a sample holding chamber fluidically connected to the inlet, and two or more metering chambers. Each metering chamber may comprise a sample inlet, a sample outlet, and a metered outlet for dispensing a predetermined volume. At least one sample distribution channel is connected between the sample outlet of a metering chamber with a sample inlet of another metering chamber. For each metering chamber, a connecting tube fluidically connects the sample inlet with the sample holding chamber, a microfluidic structure for processing the sample into a processed sample connects to the sample outlet, and a measurement structure fluidically connects to the microfluidic structure and enables measurement of the processed sample to determine the amount of the analyte in the processed sample.

Rotatable cartridge with multiple metering chambers

Rotatable cartridge for processing and analyzing a biological sample

System and method of determining an amount of an analyte in a blood sample using a cartridge and blood collector are disclosed. The blood collector has a mounting surface, a capillary structure with a curved portion, and a capillary inlet. The cartridge has a receiving surface, a cartridge inlet, a microfluidic structure, and a measurement structure. The method includes placing the blood sample into the capillary inlet; attaching the mounting surface to the receiving surface; rotating the cartridge about a rotational axis to transport the blood sample from the capillary structure to the cartridge inlet and into the microfluidic structure; controlling the rotation of the cartridge to process the blood sample into the processed sample using the microfluidic structure; controlling the rotation of the cartridge to transfer the processed sample to the measurement structure; and measuring the amount of the analyte using the measurement structure and a measurement system.

Blood collector with capillary structure

An automatic analyzer cartridge, spinnable around a rotational axis, has aliquoting and metering chambers, a connecting duct there between, and a vent connected to the metering chamber and nearer to the rotational axis than the metering chamber. The metering chamber has side walls that taper away from a central region. Capillary action next to the side walls is greater than in the central region. A circular arc about the rotational axis passes through a duct entrance in the aliquoting chamber and a duct exit in the metering chamber. The cartridge has a downstream fluidic element which is part of a fluidic structure for processing a biological sample into the processed biological sample. A valve connects the metering chamber to the fluidic element, which is fluidically connected to the fluidic structure. The fluidic structure receives the biological sample and has a measurement structure for enabling measurement of the processed biological sample.

Rotatable cartridge with a metering chamber for analyzing a biological sample

A micro flow filtration system comprises a fluid circuitry () and a first reservoir () outside the circuitry () suitable for containing a fluid. The fluid circuitry () comprises a tangential flow filtration module () capable of separating the fluid sample into a retentate stream and a permeate stream upon passage of the fluid sample into the tangential flow filtration module () through an inlet feed (). The fluid circuitry () further comprises a second reservoir () integrated in the fluid circuitry (), a pump () for creating and driving a fluid flow, optionally at least one pressure sensor (or) for acquiring and detecting data about the fluid sample, optionally a pressure regulator () for regulating the flow in the fluid circuitry () and a plurality of conduits () forming the fluid circuitry () together with the second reservoir (), the TFF-module (), the pump (), the pressure sensor (or) (if present) and the pressure regulator () (if present). The volume of the first reservoir () outside the circuitry () is significantly larger than the volume of the second reservoir (). The first reservoir () outside the fluid circuitry () is connected to the circuitry () via a connection conduit () such that the fluid flows unidirectionally into the circuitry () until the first reservoir () is empty so that a continuous fluid flow from the first reservoir () to the circuitry () is established.

Micro flow filtration system and flow filtration method

An automatic analyzer cartridge, spinnable around a rotational axis, has a support structure with a front face perpendicular to the rotational axis, a fluidic structure for processing a biological sample into the processed biological sample, a measurement structure with at least one detection zone on the front face, and a rotatable lid covering the front face. The rotatable lid is rotatable about the rotational axis relative to the support structure from a first position relative to the support structure to a second position relative to the support structure. The rotatable lid has a sample inlet opening and a detection zone opening. In the first position, a sample inlet is aligned with the sample inlet opening and the measurement structure is covered by the rotatable lid. In the second position, the sample inlet is covered by the rotatable lid and the measurement structure is aligned with the detection zone opening.

Cartridge with a rotatable lid

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Neustadt, Germany

Sascha Lutz