Promega Corporation

Sarah Duellman

Wenhui Zhou

James J Cali

Jolanta Vidugiriene

Dieter Klaubert

Hui Wang

John William Shultz

Donna M Leippe

Martha A O'Brien

Natasha Karassina

Mary Sobol

Poncho Meisenheimer

Matthew B Robers

Chad Zimprich

Joel R Walker

Peter Hofsteen

Dongping Ma

Dana Mustafa

Helene A Benink

Martha O'BRIEN

Donna Leippe

Provided herein are materials and methods for performing bioluminescent assays using a bifunctional probe. In particular, the present disclosure provides compositions and methods for detecting and/or quantifying a biomolecule and/or assaying a cellular process associated with the biomolecule using a bifunctional probe capable of binding the biomolecule and generating a bioluminescent and/or fluorescent signal.

Compositions and methods for bioluminescent detection using multifunctional probes

Conjugates comprise a drug or probe, a cell binding agent, and a quinone-containing linker. The quinone-containing linker may be reduced intracellularly to trigger release of the drug or probe. These conjugates may selectively deliver a drug or probe to a site of action of interest for local release of the active drug or probe.

Quinone-containing conjugates

Provided herein are methods for the real-time monitoring of an intracellular event or response. In particular, the methods provided herein monitor the conversion of a pro-substrate to a substrate for a protein sensor as a result of an intracellular event or response.

Real-time monitoring

Thienopyrrole compounds that may inhibit-derived luciferases are disclosed, as well as compositions and kits comprising the thienopyrrole compounds, and methods of using the thienopyrrole compounds.

Thienopyrrole compounds and uses thereof

The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.

Compounds and methods for assaying redox state of metabolically active cells and methods for measuring NAD(P)/NAD(P)H

Provided are labeling reagents and methods of using the reagents for cell uptake measurements. The labeling reagents can be quinone-masked probes including fluorophores and/or luminophores.

Quinone-masked probes as labeling reagents for cell uptake measurements

Growing community of inventors

Fitchburg, WI, United States of America

Sarah Duellman