Council of Scientific and Industrial Research

Santosh Daya Makhijani

Rita Kumar

Alka Sharma

Anil Kumar

Sharad Vishwanath Gangal

Shikha Rastogi

A Manoharan

The invention relates to a microbial composition for the neutralization of alkaline waste waters by biological means and a method of neutralization of alkaline waste waters using a synergistic mixture of the bacterial strains ofandsp.

Microbial composition and a process useful for the neutralization of alkaline waste-waters

An immobilized microbial consortium is formulated which comprises of a synergistic mixture of isolated bacteria namely, and The formulated microbial consortium is immobilized on charged nylon membrane. The said immobilized microbial consortium is attached to dissolved oxygen probe for the preparation of electrode assembly. The prepared electrode assembly is used for rapid and reliable BOD estimation. The prepared electrode assembly is used for monitoring of BOD load of synthetic samples such as Glucose-Glutamic acid (GGA) used as a reference standard in BOD analysis and industrial effluents; covering a range from low to high biodegradable organic matter.

Immobilized microbial consortium useful for rapid and reliable BOD estimation

An immobilized microbial consortium is formulated which comprises of a synergistic mixture of isolated bacteria namely, and . The formulated microbial consortium is immobilized on charged nylon membrane. The said immobilized microbial consortium is attached to dissolved oxygen probe for the preparation of electrode assembly. The prepared electrode assembly is used for rapid and reliable BOD estimation. The prepared electrode assembly is used for monitoring of BOD load of synthetic samples such as Glucose-Glutamic acid (GGA) used as a reference standard in BOD analysis and industrial effluents; covering a range from low to high biodegradable organic matter.

A reusable immobilized microbial composition is formulated. The formulated microbial composition comprises a synergistic mixture of the bacterial strains of Enterobacter cloaca, Citrobacter amalonaticus, Pseudomonas aeruginosa,Yersinia enterocolitica, Klebsiella oxytoca, Enterobacter sakazaki and Serratia liquefaciens. The formulated microbial composition is immobilized on an appropriate immobilizing agent to form beads. The said beads are tested as microbial seeding material for BOD analysis using Glucose Glutamic Acid (GGA) as a reference standard. The obtained BOD values by the formulated beads are compared with BOD values obtained by sewage as seeding material using synthetic samples as well as industrial effluents. The formulated microbial beads are ready-to-use as well as reusable seeding material in BOD analysis. The said beads can be reused up to five times with same efficacy.

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Delhi, India

Santosh Daya Makhijani