Novozymes A/s

Novo Nordisk A/s

Sanne O Schr&oslash;der Glad

Kirk Matthew Schnorr

Lene Nonboe Andersen

Markus Sakari Kauppinen

Lars Kongsbak

Martin Sch&uuml;lein

Mads Eskelund Bj&oslash;rnvad

Niels Erik Krebs Lange

S&oslash;ren M&oslash;ller

Carsten Schau Andersen

Martin Schulein

Torben Peter Frandsen

A variant of a cell-wall degrading enzyme having a beta-helix structure, which variant holds at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterising the stack as interior or exterior; characterising the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variant of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, D141 or E141, D145, K165, R194 and R199 when aligned with the pectate lyase comprising the amino acid sequence of SEQ ID NO: 2 are preferred.

Cell-wall degrading enzyme variants

A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.

Pectate lyases

A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase are derivable from Bacillus species.

Growing community of inventors

Ballerup, Denmark

Sanne O Schrøder Glad

Growing community of inventors

Ballerup, Denmark

Sanne O Schr&oslash;der Glad

Sanne O Schrøder Glad